Immunotherapeutic potential of collagen V oral administration in mBSA/CFA-induced arthritis

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Ramos da Silveira, Lizandre Keren ; Velosa, Ana Paula P. ; Catanozi, Sergio ; Pereira, Marco Aurelio A. ; dos Santos Filho, Antonio ; Marques, Fabio Luiz N. ; de Paula Faria, Daniele ; Real, Caroline Cristiano ; Fernezlian, Sandra de M. ; Yanke, Amanda Flores ; Queiroz, Zelita Aparecida de J. ; Contini, Vitoria Elias ; de Matos Lobo, Thays ; Carrasco, Solange ; Baldavira, Camila Machado ; Goldenstein-Schainberg, Claudia ; Fuller, Ricardo ; Capelozzi, Vera L. ; Teodoro, Walcy R.

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 19, n. 10, p. 15-pg., 2024-10-08.

Resumo

We hypothesized that after synovial injury, collagen V (Col V) expose occult antigens, and Col V autoantibodies develop, indicating the loss of immune tolerance against this molecule, thus leading to damage to mesenchymal-derived cells as well as the extracellular matrix in experimental arthritis. Thus, the present study investigated the effects of oral administration of Col V on the synovium after the development of inflammation in mBSA/CFA-induced arthritis. After fourteen days of intraarticular administration of mBSA, 10 male Lewis rats were orally administered Col V (500 mu g/300 mu L) diluted in 0.01 N acetic acid (IA-Col V group). The arthritic group (IA group, n = 10) received only intraarticular mBSA. An intra-articular saline injection (20 mu L) was given to the control group (CT-Col V, n = 5). IA group presented damaged synovia, the expansion of the extracellular matrix by cellular infiltrate, which was characterized by T and B lymphocytes, and fibroblastic infiltration. In contrast, after Col V oral immunotherapy IA-Col V group showed a significant reduction in synovial inflammation and intense expression of IL-10+ and FoxP3+ cells, in addition to a reduction in Col V and an increase in Col I in the synovia compared to those in the IA group. Furthermore, an increase in IL-10 production was detected after IA-Col V group spleen cell stimulation with Col V in vitro. PET imaging did not differ between the groups. The evaluation of oral treatment with Col V, after mBSA/CFA-induced arthritis in rats, protects against inflammation and reduces synovial tissue damage, through modulation of the synovial matrix, showing an immunotherapeutic potential in inhibiting synovitis. (AU)

• Processo FAPESP: 19/24178-0 - Efeito da suplementação do colágeno tipo V no processo inflamatório e remodelamento da matriz extracelular na artrite experimental
• Beneficiário: Lizandre Keren Ramos da Silveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 23/02755-0 - Avaliação da expressão proteica da transição epitélio-mesenquimal como nova perspectiva em biomarcadores para a determinação do prognóstico, resposta e resistência à terapia alvo nos carcinomas pulmonares não pequenas células
• Beneficiário: Camila Machado Baldavira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/20403-6 - Marcadores biomoleculares de proliferação e remodelamento em doenças respiratórias agudas e crônicas: promissores alvos terapêuticos
• Beneficiário: Vera Luiza Capelozzi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/13220-5 - Efeito da indução de tolerância com peptídeos imunogênicos do colágeno tipo V na fibrose pulmonar associada à esclerose sistêmica experimental
• Beneficiário: Ana Paula Pereira Velosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular