| Texto completo | |
| Autor(es): Mostrar menos - |
Ramos da Silveira, Lizandre Keren
;
Velosa, Ana Paula P.
;
Catanozi, Sergio
;
Pereira, Marco Aurelio A.
;
dos Santos Filho, Antonio
;
Marques, Fabio Luiz N.
;
de Paula Faria, Daniele
;
Real, Caroline Cristiano
;
Fernezlian, Sandra de M.
;
Yanke, Amanda Flores
;
Queiroz, Zelita Aparecida de J.
;
Contini, Vitoria Elias
;
de Matos Lobo, Thays
;
Carrasco, Solange
;
Baldavira, Camila Machado
;
Goldenstein-Schainberg, Claudia
;
Fuller, Ricardo
;
Capelozzi, Vera L.
;
Teodoro, Walcy R.
Número total de Autores: 19
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 19, n. 10, p. 15-pg., 2024-10-08. |
| Resumo | |
We hypothesized that after synovial injury, collagen V (Col V) expose occult antigens, and Col V autoantibodies develop, indicating the loss of immune tolerance against this molecule, thus leading to damage to mesenchymal-derived cells as well as the extracellular matrix in experimental arthritis. Thus, the present study investigated the effects of oral administration of Col V on the synovium after the development of inflammation in mBSA/CFA-induced arthritis. After fourteen days of intraarticular administration of mBSA, 10 male Lewis rats were orally administered Col V (500 mu g/300 mu L) diluted in 0.01 N acetic acid (IA-Col V group). The arthritic group (IA group, n = 10) received only intraarticular mBSA. An intra-articular saline injection (20 mu L) was given to the control group (CT-Col V, n = 5). IA group presented damaged synovia, the expansion of the extracellular matrix by cellular infiltrate, which was characterized by T and B lymphocytes, and fibroblastic infiltration. In contrast, after Col V oral immunotherapy IA-Col V group showed a significant reduction in synovial inflammation and intense expression of IL-10+ and FoxP3+ cells, in addition to a reduction in Col V and an increase in Col I in the synovia compared to those in the IA group. Furthermore, an increase in IL-10 production was detected after IA-Col V group spleen cell stimulation with Col V in vitro. PET imaging did not differ between the groups. The evaluation of oral treatment with Col V, after mBSA/CFA-induced arthritis in rats, protects against inflammation and reduces synovial tissue damage, through modulation of the synovial matrix, showing an immunotherapeutic potential in inhibiting synovitis. (AU) | |
| Processo FAPESP: | 19/24178-0 - Efeito da suplementação do colágeno tipo V no processo inflamatório e remodelamento da matriz extracelular na artrite experimental |
| Beneficiário: | Lizandre Keren Ramos da Silveira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 23/02755-0 - Avaliação da expressão proteica da transição epitélio-mesenquimal como nova perspectiva em biomarcadores para a determinação do prognóstico, resposta e resistência à terapia alvo nos carcinomas pulmonares não pequenas células |
| Beneficiário: | Camila Machado Baldavira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/20403-6 - Marcadores biomoleculares de proliferação e remodelamento em doenças respiratórias agudas e crônicas: promissores alvos terapêuticos |
| Beneficiário: | Vera Luiza Capelozzi |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/13220-5 - Efeito da indução de tolerância com peptídeos imunogênicos do colágeno tipo V na fibrose pulmonar associada à esclerose sistêmica experimental |
| Beneficiário: | Ana Paula Pereira Velosa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |