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| Autor(es): Mostrar menos - |
Delafiori, Jeany
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Siciliano, Rinaldo Focaccia
;
de Oliveira, Arthur Noin
;
Nicolau, Jose Carlos
;
Sales, Geovana Manzan
;
Dalcoquio, Talia Falcao
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Busanello, Estela Natacha Brandt
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Eguti, Adriana
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de Oliveira, Diogo Noin
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Bertolin, Adriadne Justi
;
dos Santos, Luiz Augusto
;
Salsoso, Rocio
;
Marcondes-Braga, Fabiana G.
;
Duran, Nelson
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Perroud Jr, Mauricio Wesley
;
Sabino, Ester Cerdeira
;
Reis, Leonardo Oliveira
;
Favaro, Wagner Jose
;
Catharino, Rodrigo Ramos
Número total de Autores: 19
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| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF MOLECULAR MEDICINE-JMM; v. 102, n. 2, p. 13-pg., 2023-11-27. |
| Resumo | |
As SARS-CoV-2 continues to produce new variants, the demand for diagnostics and a better understanding of COVID-19 remain key topics in healthcare. Skin manifestations have been widely reported in cases of COVID-19, but the mechanisms and markers of these symptoms are poorly described. In this cross-sectional study, 101 patients (64 COVID-19 positive patients and 37 controls) were enrolled between April and June 2020, during the first wave of COVID-19, in Sao Paulo, Brazil. Enrolled patients had skin imprints sampled non-invasively using silica plates; plasma samples were also collected. Samples were used for untargeted lipidomics/metabolomics through high-resolution mass spectrometry. We identified 558 molecular ions, with lipids comprising most of them. We found 245 plasma ions that were significant for COVID-19 diagnosis, compared to 61 from the skin imprints. Plasma samples outperformed skin imprints in distinguishing patients with COVID-19 from controls, with F1-scores of 91.9% and 84.3%, respectively. Skin imprints were excellent for assessing disease severity, exhibiting an F1-score of 93.5% when discriminating between patient hospitalization and home care statuses. Specifically, oleamide and linoleamide were the most discriminative biomarkers for identifying hospitalized patients through skin imprinting, and palmitic amides and N-acylethanolamine 18:0 were also identified as significant biomarkers. These observations underscore the importance of primary fatty acid amides and N-acylethanolamines in immunomodulatory processes and metabolic disorders. These findings confirm the potential utility of skin imprinting as a valuable non-invasive sampling method for COVID-19 screening; a method that may also be applied in the evaluation of other medical conditions.Key messagesSkin imprints complement plasma in disease metabolomics.The annotated markers have a role in immunomodulation and metabolic diseases.Skin imprints outperformed plasma samples at assessing disease severity.Skin imprints have potential as non-invasive sampling strategy for COVID-19. (AU) | |
| Processo FAPESP: | 20/04705-2 - Estudo diagnóstico e prognóstico de infecção por SARS-CoV-2 e Influenza vírus |
| Beneficiário: | José Carlos Nicolau |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/10052-1 - Novas Abordagens Terapêuticas para o Câncer de Bexiga Não-Músculo Invasivo (CBNMI): Uso Intravesical do Modificador de Resposta Biológica OncoTherad e Sua Associação com o Plasma Rico em Plaquetas (PRP) |
| Beneficiário: | Wagner José Fávaro |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/05718-3 - Determinação das alterações metabólicas e do potencial terapêutico do Zika Vírus em células tumorais por espectrometria de massas e inteligência artificial |
| Beneficiário: | Jeany Delafiori |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |