Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection

Autor(es)

Alvarado-Mora, Monica V. ^{[1, 2]} ; Botelho, Livia ^{[1, 2]} ; Nishiya, Anna ^[3] ; Neto, Raymundo A. ^[4] ; Gomes-Gouvea, Michele S. ^{[1, 2]} ; Gutierrez, Maria F. ^[5] ; Carrilho, Flair J. ^{[1, 2]} ; Pinho, Joao R. R. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Gastroenterol & Hepatol, Sao Paulo Inst Trop Med, Sao Paulo - Brazil

^[3] Sao Paulo Blood Bank, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[5] Pontificia Javeriana Univ, Virol Lab, Dept Microbiol, Bogota - Colombia

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 8, JUL 11 2011.
• Citações Web of Science: 9

Resumo

Background: GB virus C (GBV-C) is an enveloped positive-sense ssRNA virus belonging to the Flaviviridae family. Studies on the genetic variability of the GBV-C reveals the existence of six genotypes: genotype 1 predominates in West Africa, genotype 2 in Europe and America, genotype 3 in Asia, genotype 4 in Southwest Asia, genotype 5 in South Africa and genotype 6 in Indonesia. The aim of this study was to determine the frequency and genotypic distribution of GBV-C in the Colombian population. Methods: Two groups were analyzed: i) 408 Colombian blood donors infected with HCV (n = 250) and HBV (n = 158) from Bogota and ii) 99 indigenous people with HBV infection from Leticia, Amazonas. A fragment of 344 bp from the 5' untranslated region (5' UTR) was amplified by nested RT PCR. Viral sequences were genotyped by phylogenetic analysis using reference sequences from each genotype obtained from GenBank (n = 160). Bayesian phylogenetic analyses were conducted using Markov chain Monte Carlo (MCMC) approach to obtain the MCC tree using BEAST v. 1.5.3. Results: Among blood donors, from 158 HBsAg positive samples, eight 5.06% (n = 8) were positive for GBV-C and from 250 anti-HCV positive samples, 3.2%(n = 8) were positive for GBV-C. Also, 7.7% (n = 7) GBV-C positive samples were found among indigenous people from Leticia. A phylogenetic analysis revealed the presence of the following GBV-C genotypes among blood donors: 2a (41.6%), 1 (33.3%), 3 (16.6%) and 2b (8.3%). All genotype 1 sequences were found in co-infection with HBV and 4/5 sequences genotype 2a were found in co-infection with HCV. All sequences from indigenous people from Leticia were classified as genotype 3. The presence of GBV-C infection was not correlated with the sex (p = 0.43), age (p = 0.38) or origin (p = 0.17). Conclusions: It was found a high frequency of GBV-C genotype 1 and 2 in blood donors. The presence of genotype 3 in indigenous population was previously reported from Santa Marta region in Colombia and in native people from Venezuela and Bolivia. This fact may be correlated to the ancient movements of Asian people to South America a long time ago. (AU)

• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular