Autor(es)

Araujo, Layanne C. C. ; Dias, Carolina C. B. ; Sucupira, Felipe G. ; Ramalho, Leandra N. Z. ; Camporez, Joao Paulo

• Tipo de documento: Artigo Científico
• Fonte: BIOSCIENCE REPORTS; v. 44, n. 5, p. 11-pg., 2024-05-01.

Resumo

Several models of mice-fed high -fat diets have been used to trigger non-alcoholic steatohepatitis and some chemical substances, such as carbon tetrachloride. The present study aimed to evaluate the joint action of a high -fat diet and CCl 4 in developing a short-term non-alcoholic steatohepatitis model. C57BL6/J mice were divided into two groups: standard diet-fed (SD), the high -fat diet-fed (HFD) and HFD + fructose-fed and carbon tetrachloride (HFD+CCl 4 ). The animals fed with HFD+CCl 4 presented increased lipid deposition compared with both SD and HFD mice. Plasma cholesterol was increased in animals from the HFD+CCl 4 group compared with the SD and HFD groups, without significant differences between the SD and HFD groups. Plasma triglycerides showed no significant difference between the groups. The HFD+CCl 4 animals had increased collagen deposition in the liver compared with both SD and HFD groups. Hydroxyproline was also increased in the HFD+CCl 4 group. Liver enzymes, alanine aminotransferase and aspartate aminotransferase, were increased in the HFD+CCl 4 group, compared with SD and HFD groups. Also, CCl 4 was able to trigger an inflammatory process in the liver of HFD-fed animals by promoting an increase of -2 times in macrophage activity, -6 times in F4/80 gene expression, and pro-inflammatory cytokines (IL -1b and TNFa), in addition to an increase in inflammatory pathway protein phosphorylation (IKKbp). HFD e HFD+CCl 4 animals increased glucose intolerance compared with SD mice, associated with reduced insulin-stimulated AKT activity in the liver. Therefore, our study has shown that short-term HFD feeding associated with fructose and CCl 4 can trigger non-alcoholic steatohepatitis and cause damage to glucose metabolism. (AU)

• Processo FAPESP: 22/05445-0 - Desenvolvimento e caracterização de um novo modelo de Esteato-Hepatite Não Alcoólica em camundongos
• Beneficiário: Carolina Coelho Barretto Dias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/04956-5 - Impacto do receptor de estrogênio alpha na Doença Hepática Gordurosa não Alcoólica e metabolismo energético do fígado
• Beneficiário: João Paulo Gabriel Camporez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 20/09094-1 - Impacto da inibição de galectina-3 sobre a resistência hepática à insulina e metabolismo energético na Esteato-Hepatite Não Alcoólica
• Beneficiário: Layanne Cabral da Cunha Araujo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/02638-9 - Impacto do receptor de estrogênio alpha na doença hepática gordurosa não alcoólica e metabolismo energético do fígado
• Beneficiário: Felipe Garcia da Silva Sucupira
• Modalidade de apoio: Bolsas no Brasil - Mestrado