| Texto completo | |
| Autor(es): |
Alvares, Dayane S.
;
Monti, Mariela R.
;
Neto, Joao Ruggiero
;
Wilke, Natalia
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BBA ADVANCES; v. 1, p. 11-pg., 2021-01-01. |
| Resumo | |
Polybia-MP1 is an antimicrobial peptide that shows a decreased activity in membranes with cholesterol (CHO). Since it is now accepted that hopanoids act as sterol-surrogates in some sterol-lacking bacteria, we here inquire about the impact of Polybia-MP1 on membranes containing the hopanoid diplopterol (DP) in comparison to membranes with CHO. We found that, despite the properties induced on lipid membranes by DP are similar to those induced by CHO, the effect of Polybia-MP1 on membranes with CHO or DP was significantly different. DP did not prevent dye release from LUVs, nor the insertion of Polybia-MP1 into monolayers, and peptide-membrane affinity was higher for those with DP than with CHO. Zeta potentials (zeta) for DP-containing LUVs showed a complex behavior at increasing peptide concentration. The effect of the peptide on membrane elasticity, investigated by nanotube retraction experiments, showed that peptide addition softened all membrane compositions, but membranes with DP got stiffer at long times. Considering this, and the zeta results, we propose that peptides accumulate at the interface adopting different arrangements, leading to a non-monotonic behavior. Possible correlations with cell membranes were inquired testing the antimicrobial activity of Polybia-MP1 against hopanoid-lacking bacteria pre-incubated with DP or CHO. The fraction of surviving cells was lower in cultures incubated with DP compared to those incubated with CHO. We propose that the higher activity of Polybia-MP1 against some bacteria compared to mammalian cells is not only related to membrane electrostatics, but also the composition of neutral lipids, particularly the hopanoids, could be important. (AU) | |
| Processo FAPESP: | 15/25619-9 - Efeito de aminofosfolípides e do pH na atividade interfacial do peptídeo anticâncer Polybia-MP1 e análogos em membranas modelo |
| Beneficiário: | João Ruggiero Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/25620-7 - Interação membrana/peptídeo: propriedades mecânica e eletrostática em sistemas com domínios lipídicos |
| Beneficiário: | Dayane dos Santos Alvares |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/14215-2 - Regulação da atividade de peptídeos antimicrobianos pelas propriedades mecânicas da membrana alvo |
| Beneficiário: | Dayane dos Santos Alvares |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |