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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photodegradation of Folate Sensitized by Riboflavin

Texto completo
Autor(es):
Scurachio, Regina S. [1] ; Skibsted, Leif H. [2] ; Metzker, Gustavo [1] ; Cardoso, Daniel R. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Paulo - Brazil
[2] Univ Copenhagen, Dept Food Sci, Fac Life Sci, Frederiksberg C - Denmark
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Photochemistry and Photobiology; v. 87, n. 4, p. 840-845, JUL-AUG 2011.
Citações Web of Science: 12
Resumo

Folate is shown to react with singlet-excited state of riboflavin in a diffusion controlled reaction and with triplet-excited state of riboflavin in a somewhat slower reaction with (3)k(q) = 4.8 x 10(8) L mol(-1) s(-1) in aqueous phosphate buffer at pH 7.4, ionic strength of 0.2 mol L(-1), and 25 degrees C. Singlet quenching is assigned as photo-induced reductive electron transfer from ground state folate to singlet-excited riboflavin, while triplet quenching is assigned as one-electron transfer rather than hydrogen atom transfer from folate to triplet-excited riboflavin, as the reaction quantum yield, phi = 0.32, is hardly influenced by solvent change from water to deuterium oxide, phi = 0.37. Cyclic voltammetry showed an irreversible two-electron anodic process for folate, E = 1.14 V versus NHE at a scan-rate of 50 mV s(-1), which appears to be kinetically controlled by the heterogeneous electron transfer from the substrates to the electrode. Main products of folate photooxidation sensitized by riboflavin were pterin-6-carboxylic acid and p-aminobenzoyl-L-glutamic acid as shown by liquid chromatographic ion-trap mass spectrometry (LC-IT-MS). (AU)

Processo FAPESP: 09/00858-0 - Mecanismo e conseqüências de processos redox em alimentos: aspectos químicos e fotoquímicos da deterioração oxidativa e atividade antioxidante de cerveja e produtos lácteos
Beneficiário:Daniel Rodrigues Cardoso
Modalidade de apoio: Auxílio à Pesquisa - Regular