Autor(es)

Grajales, Lina Maria ; Wang, Hailei ; Casciatori, Fernanda Perpetua ; Thomeo, Joao Claudio

• Tipo de documento: Artigo Científico
• Fonte: CHEMICAL ENGINEERING AND PROCESSING; v. 210, p. 11-pg., 2025-04-01.

Resumo

Cellulolytic enzymes are vital for converting cellulosic residues into biofuels, yet large-scale production through solid-state cultivation (SSC) remains challenging due to the lack of suitable bioreactors. This study addresses this issue by developing a rotary drum bioreactor to produce cellulases from the thermophilic fungus Myceliophthora thermophila I-1D3b, using sugarcane bagasse and wheat bran as substrates. The bioreactor integrates upstream, fermentation, and downstream processes, streamlining production and enhancing efficiency. The study explored enzymatic activity (EA) at varying substrate loadings and drum rotation conditions. Although statistically similar, at 50 % loading, drum rotation slightly improved EA (49.12 U/mL f 6.56 U/mL) compared to static conditions (47.78 U/mL f 8.25 U/mL). Conversely, at 40 % loading, rotation reduced EA significantly (23.57 U/ mL f 3.17 U/mL) compared to static conditions (46.91 U/mL f 8.17 U/mL). At 60 % loading, EA was similar under both static and rotated conditions. The design effectively supports fermentation, facilitates enzymatic extract recovery, and minimizes temperature and moisture gradients. These results demonstrate the rotary drum bioreactor's potential for scaling up cellulase production, offering a promising solution for industrial SSC processes. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 18/00996-2 - Desenvolvimento de um biorreator de leito empacotado pseudo-contínuo para fermentação em estado sólido
• Beneficiário: Fernanda Perpétua Casciatori
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 14/23453-3 - Ampliação de escala de biorreatores de leito empacotado para a produção de celulases por fermentação em estado sólido
• Beneficiário: João Cláudio Thoméo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular