| Texto completo | |
| Autor(es): Mostrar menos - |
Breunig, Kira
;
Lei, Xuifen
;
Montalbano, Mauro
;
Guardia, Gabriela D. A.
;
Ostadrahimi, Shiva
;
Alers, Victoria
;
Kosti, Adam
;
Chiou, Jennifer
;
Klein, Nicole
;
Vinarov, Corina
;
Wang, Lily
;
Li, Mujia
;
Song, Weidan
;
Kraus, W. Lee
;
Libich, David S.
;
Tiziani, Stefano
;
Weintraub, Susan T.
;
Galante, Pedro A. F.
;
Penalva, Luiz O.
Número total de Autores: 19
|
| Tipo de documento: | Artigo Científico |
| Fonte: | eLIFE; v. 13, p. 37-pg., 2025-02-12. |
| Resumo | |
RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such as stress granules and nucleoli. IDR-RBPs are particularly relevant in the nervous system and their dysfunction is associated with neurodegenerative diseases and brain tumor development. Serpine1 mRNA-binding protein 1 (SERBP1) is a unique member of this group, being mostly disordered and lacking canonical RNA-binding domains. We defined SERBP1's interactome, uncovered novel roles in splicing, cell division and ribosomal biogenesis, and showed its participation in pathological stress granules and Tau aggregates in Alzheimer's brains. SERBP1 preferentially interacts with other G-quadruplex (G4) binders, implicated in different stages of gene expression, suggesting that G4 binding is a critical component of SERBP1 function in different settings. Similarly, we identified important associations between SERBP1 and PARP1/polyADP-ribosylation (PARylation). SERBP1 interacts with PARP1 and its associated factors and influences PARylation. Moreover, protein complexes in which SERBP1 participates contain mostly PARylated proteins and PAR binders. Based on these results, we propose a feedback regulatory model in which SERBP1 influences PARP1 function and PARylation, while PARylation modulates SERBP1 functions and participation in regulatory complexes. (AU) | |
| Processo FAPESP: | 17/19541-2 - Impacto de proteínas de ligação a RNA na desregulação de splicing em glioblastoma |
| Beneficiário: | Gabriela Der Agopian Guardia |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/15579-8 - Retroelementos: uma força motriz criando novidades genéticas no genoma humano e de camundongos |
| Beneficiário: | Pedro Alexandre Favoretto Galante |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |