Nose-to-Brain Delivery of Biomimetic Nanoparticles for Glioblastoma Targeted Therapy

Autor(es)

Ferreira, Natalia Noronha ; Leite, Celisnolia Morais ; Moreno, Natalia Sanchez ; Miranda, Renata Rank ; Lins, Paula Maria Pincela ; Rodero, Camila Fernanda ; de Oliveira Junior, Edilson ; Lima, Eliana Martins ; Reis, Rui M. ; Zucolotto, Valtencir

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 17, n. 1, p. 16-pg., 2024-12-18.

Resumo

Glioblastoma (GBM) is an extremely aggressive form of brain cancer that remains challenging to treat, especially owing to the lack of effective targeting and drug delivery concerns. Due to its anatomical advantages, the nose-to-brain strategy is an interesting route for drug delivery. Nanoengineering has provided technological tools and innovative strategies to overcome biotechnological limitations, which is promising for improving the effectiveness of conventional therapies. Herein, we designed a biomimetic multifunctional nanostructure produced by polymeric poly(d,l-lactic-co-glycolic) acid (PLGA) core loaded with Temozolomide (TMZ) coated with cell membrane isolated from glioma cancer cells. The developed nanostructures (NP-MB) were fully characterized, and their biological performance was investigated extensively. The results indicate that NP-MB could control TMZ release and promote TMZ permeation in the ex vivo nasal porcine mucosa. The higher cytotoxicity of NP-MB in different glioma cell lines, particularly against U251 cells, reinforces their potential for homotypic targeting. The chicken chorioallantoic membrane assay revealed a tumor size reduction and antiangiogenic activity. In vivo biodistribution studies showed that NP-MB effectively reaches the brain following nasal administration. These findings suggest that NP-MB holds promise as a biomimetic nanoplatform for effective targeting and homotypic recognition in GBM therapy with high potential for clinical translation. (AU)

• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/25645-0 - Sistemas nanostruturados bioinspirados e biomiméticos para administração via nasal: uma nova perspectiva para a terapia de glioblastoma
• Beneficiário: Natália Noronha Ferreira Naddeo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/00124-5 - Nanomateriais funcionalizados para aplicação em terapias contra o câncer
• Beneficiário: Valtencir Zucolotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular