6-Nitrodopamine potentiates catecholamine-induced contractions of human isolated vas deferens

Autor(es)

Lima, Antonio Tiago ; Jabbour, Sami ; Britto-Junior, Jose ; de Carvalho, Demetrio Martinho Ramos ; Fregonesi, Adriano ; Mariano, Fernanda V. ; de Souza, Valeria Barbosa ; Schenka, Andre Almeida ; Antunes, Edson ; De Nucci, Gilberto

• Tipo de documento: Artigo Científico
• Fonte: FASEB BIOADVANCES; v. 7, n. 2, p. 9-pg., 2025-01-15.

Resumo

6-Nitrodopamine (6-ND) is the main catecholamine released from human isolated vas deferens and the adrenergic nervous system is known to play a major role in the contractions of the epididymal portion of the vas deferens. Here it was investigated the interactions of 6-ND on the contractions of the human isolated vas deferens induced by either classical catecholamines or electric-field stimulation (EFS). The vas deferens obtained from 106 patients who underwent vasectomy surgery were mounted in a 10-mL glass chamber filled with warmed (37 degrees C) and oxygenated Krebs-Henseleit's solution. The strips were pretreated (30 min) with 6-ND (0.1-100 nM) and exposed to increasing concentrations of noradrenaline (0.01-300 M), dopamine (0.00001-10 mM), or adrenaline (0.01-300 M). The strips were also submitted to EFS in tissues pre-incubated or not with 6-ND (1-100 nM), noradrenaline (100 nM), adrenaline (100 nM), or dopamine (100 nM). Catecholamine basal release was evaluated by LC-MS/MS and expression of tyrosine hydroxylase by both immunohistochemistry (IC) and fluorescence in-situ hybridization (FISH). Pre-incubation of the vas deferens with 6-ND caused marked potentiation of the contractions induced by noradrenaline, adrenaline, and dopamine, as characterized by significant increases in Emax, without changes in pEC50 values. 6-nitrodopamine also caused significant increases in the EFS-induced contractions. The basal release of 6-ND was not affected by pre-treatment of the tissues with tetrodotoxin. Tyrosine hydroxylase was detected in epithelial cells of human vas deferens samples by both IC and FISH. The results clearly demonstrate that epithelium-derived 6-ND is a major modulator of human vas deferens contractility. (AU)

• Processo FAPESP: 19/16805-4 - Avaliação do papel fisiopatológico das catecolaminas endoteliais
• Beneficiário: Gilberto de Nucci
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata
• Beneficiário: Edson Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/13593-6 - Avaliação do tratamento crônico da 6-nitrodopamina em ratos
• Beneficiário: Antonio Tiago Silva Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 21/14414-8 - Avaliação da ação das nitro-catecolaminas no sistema cardiovascular
• Beneficiário: José Britto Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/13726-6 - Identificação de moléculas relacionadas à síntese de adrenalina, noradrenalina e dopamina no endotélio de répteis e mamíferos, por meio de técnicas imuno-histoquímicas e moleculares in situ
• Beneficiário: Valéria Barbosa de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado