| Texto completo | |
| Autor(es): |
Lima, Antonio Tiago
;
Jabbour, Sami
;
Britto-Junior, Jose
;
de Carvalho, Demetrio Martinho Ramos
;
Fregonesi, Adriano
;
Mariano, Fernanda V.
;
de Souza, Valeria Barbosa
;
Schenka, Andre Almeida
;
Antunes, Edson
;
De Nucci, Gilberto
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FASEB BIOADVANCES; v. 7, n. 2, p. 9-pg., 2025-01-15. |
| Resumo | |
6-Nitrodopamine (6-ND) is the main catecholamine released from human isolated vas deferens and the adrenergic nervous system is known to play a major role in the contractions of the epididymal portion of the vas deferens. Here it was investigated the interactions of 6-ND on the contractions of the human isolated vas deferens induced by either classical catecholamines or electric-field stimulation (EFS). The vas deferens obtained from 106 patients who underwent vasectomy surgery were mounted in a 10-mL glass chamber filled with warmed (37 degrees C) and oxygenated Krebs-Henseleit's solution. The strips were pretreated (30 min) with 6-ND (0.1-100 nM) and exposed to increasing concentrations of noradrenaline (0.01-300 M), dopamine (0.00001-10 mM), or adrenaline (0.01-300 M). The strips were also submitted to EFS in tissues pre-incubated or not with 6-ND (1-100 nM), noradrenaline (100 nM), adrenaline (100 nM), or dopamine (100 nM). Catecholamine basal release was evaluated by LC-MS/MS and expression of tyrosine hydroxylase by both immunohistochemistry (IC) and fluorescence in-situ hybridization (FISH). Pre-incubation of the vas deferens with 6-ND caused marked potentiation of the contractions induced by noradrenaline, adrenaline, and dopamine, as characterized by significant increases in Emax, without changes in pEC50 values. 6-nitrodopamine also caused significant increases in the EFS-induced contractions. The basal release of 6-ND was not affected by pre-treatment of the tissues with tetrodotoxin. Tyrosine hydroxylase was detected in epithelial cells of human vas deferens samples by both IC and FISH. The results clearly demonstrate that epithelium-derived 6-ND is a major modulator of human vas deferens contractility. (AU) | |
| Processo FAPESP: | 19/16805-4 - Avaliação do papel fisiopatológico das catecolaminas endoteliais |
| Beneficiário: | Gilberto de Nucci |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata |
| Beneficiário: | Edson Antunes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/13593-6 - Avaliação do tratamento crônico da 6-nitrodopamina em ratos |
| Beneficiário: | Antonio Tiago Silva Lima |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 21/14414-8 - Avaliação da ação das nitro-catecolaminas no sistema cardiovascular |
| Beneficiário: | José Britto Júnior |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/13726-6 - Identificação de moléculas relacionadas à síntese de adrenalina, noradrenalina e dopamina no endotélio de répteis e mamíferos, por meio de técnicas imuno-histoquímicas e moleculares in situ |
| Beneficiário: | Valéria Barbosa de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |