| Texto completo | |
| Autor(es): |
Fonseca, Angelica Cristina
;
Colavite, Priscila Maria
;
Azevedo, Michelle de Campos Soriani
;
Passadori, Daniela Carignatto
;
Melchiades, Jessica Lima
;
Ortiz, Rafael Carneiro
;
Rodini, Camila Oliveira
;
Trombone, Ana Paula Favaro
;
Garlet, Gustavo Pompermaier
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOLOGY-BASEL; v. 14, n. 2, p. 22-pg., 2025-02-01. |
| Resumo | |
Dental socket repair theoretically involves a constructive inflammatory immune response, which evolves from an initial M1 prevalence to a subsequent M2 dominance. In this scenario, the MEK1/2 signaling pathway is allegedly involved in M2 polarization. This study aimed to evaluate the impact of MEK1/2 pharmacological inhibition in the local host response and repair outcome. C57Bl/6-WT 8-week-old male mice were submitted to the extraction of the right upper incisor and treated (or not, control group) with MEK1/2 inhibitor PD0325901 (10 mg/kg/24 h/IP, MEK1/2i group) and analyzed at 0, 3, 7, and 14 days using microcomputed tomography, histomorphometry, birefringence, immunohistochemistry, and PCR array analysis. The results demonstrate that MEK1/2 inhibition limits the development of M2 response over time, being associated with lower expression of M2, MSCs, and bone markers, lower levels of growth and osteogenic factors, along with a higher expression of iNOS, IL-1b, IL-6, and TNF-alpha, as well inflammatory chemokines, indicating a predominantly M1 pro-inflammatory environment. This modulation of local inflammatory immune response is associated with impaired bone formation as demonstrated by microtomographic and histomorphometric data. The results show that MEK1/2 inhibition delays bone repair after tooth extraction, supporting the concept that M2 macrophages are essential elements for host response regulation and proper repair. (AU) | |
| Processo FAPESP: | 18/10177-9 - Análise do impacto da inibição de MEK1/2 e STAT6 na polarização de macrófagos para perfil M2, e sua influência no processo de reparo alveolar pós-exodontia em camundongos. |
| Beneficiário: | Angelica Cristina Fonseca |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 15/24637-3 - MSCs e M2 como determinantes da natureza construtiva ou destrutiva de microambientes inflamatórios associados ao tecido ósseo |
| Beneficiário: | Gustavo Pompermaier Garlet |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |