| Texto completo | |
| Autor(es): |
Moro, Lincoln Gozzi
;
Guarnier, Lucas Pires
;
Azevedo, Mauricio Fogaca
;
Fracasso, Julia Amanda Rodrigues
;
Lucio, Marco Aurelio
;
de Castro, Mateus Vidigal
;
Dias, Marlon Lemos
;
Livero, Francislaine Aparecida dos Reis
;
Ribeiro-Paes, Joao Tadeu
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELLS; v. 13, n. 24, p. 24-pg., 2024-12-01. |
| Resumo | |
This comprehensive overview of the historical milestones in cell culture underscores key breakthroughs that have shaped the field over time. It begins with Wilhelm Roux's seminal experiments in the 1880s, followed by the pioneering efforts of Ross Granville Harrison, who initiated groundbreaking experiments that fundamentally shaped the landscape of cell culture in the early 20th century. Carrel's influential contributions, notably the immortalization of chicken heart cells, have marked a significant advancement in cell culture techniques. Subsequently, Johannes Holtfreter, Aron Moscona, and Joseph Leighton introduced methodological innovations in three-dimensional (3D) cell culture, initiated by Alexis Carrel, laying the groundwork for future consolidation and expansion of the use of 3D cell culture in different areas of biomedical sciences. The advent of induced pluripotent stem cells by Takahashi and Yamanaka in 2006 was revolutionary, enabling the reprogramming of differentiated cells into a pluripotent state. Since then, recent innovations have included spheroids, organoids, and organ-on-a-chip technologies, aiming to mimic the structure and function of tissues and organs in vitro, pushing the boundaries of biological modeling and disease understanding. In this review, we overview the history of cell culture shedding light on the main discoveries, pitfalls and hurdles that were overcome during the transition from 2D to 3D cell culture techniques. Finally, we discussed the future directions for cell culture research that may accelerate the development of more effective and personalized treatments. (AU) | |
| Processo FAPESP: | 23/05446-9 - Estabelecimento de um bioprocesso para produção e bioimpressão 3D de um hidrogel resultante da associação de alginato e óleo de pequi definido morfologicamente a partir de escaneamento de úlceras cutâneas virtuais |
| Beneficiário: | Maurício Fogaça Azevedo |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 20/09702-1 - Estudo de variantes genéticas protetoras em indivíduos nonagenários e centenários resilientes |
| Beneficiário: | Mateus Vidigal de Castro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 23/03687-9 - Avaliação dos efeitos citotóxicos e genotóxicos da Pereskia grandifolia Haw. e determinação da eficácia terapêutica empregando modelo de cultura celular tridimensional (3D) de fígado com Esteatose |
| Beneficiário: | Lucas Pires Guarnier |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |