| Texto completo | |
| Autor(es): |
Alberto-Silva, Carlos
;
Portaro, Fernanda Calheta Vieira
;
Kodama, Roberto Tadashi
;
Gomes, Lais
;
da Silva, Brenda Rufino
;
da Cunha e Silva, Felipe Assumpcao
;
Nihei, Ken-ichi
;
Konno, Katsuhiro
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXINS; v. 16, n. 10, p. 15-pg., 2024-10-01. |
| Resumo | |
A comprehensive LC-MS study examined the venom components of the solitary scoliid wasp Scolia oculata. Online mass fingerprinting showed that crude venom contains 25 small molecules (amino acids, biogenic amines, and nucleosides/nucleotides) and 45 peptides with MW 400-2700. The small molecules were identified by elemental composition analysis, and peptide sequences were determined by ESI-MS/MS and MALDI-TOF/TOF MS analyses. As major peptide components, a known peptide, beta-scoliidine (DYVTVKGFSPLRKA), and three new peptides, gamma-scoliidine (YVTVKGFSPLR), delta-scoliidine (YVTVKGFSPLREP) and epsilon-scoliidine (DYVTVKGFSPLREP) were identified, all of which are closely homologous to each other. Once the neuroprotective effects of beta-scoliidine have already been described, the other three new scoliidine peptides were analyzed against oxidative stress-induced toxicity in PC12 neuronal cells by mitochondrial metabolism assay, and the structure-activity relationship was evaluated. Interestingly, pre-treatment with epsilon-scoliidine increased the mitochondrial metabolism of PC12 cells (106 +/- 3.6%; p = 0.007) exposed to H2O2-induced oxidative stress in contrast to gamma- and delta-scoliidines (77.6 +/- 4.8 and 68.5 +/- 4.1%, respectively) in compared to cells treated only H2O2 (75.8 +/- 2.4%). These new peptides were also analyzed for enzyme inhibitor/substrate assays with angiotensin-converting enzyme (ACE), neprilysin (NEP), and acetylcholinesterase (AChE). In these assays, only delta- and epsilon-scoliidines increased the AChE activity (128.7 +/- 3.8%; p = 0.01; and 116.8 +/- 3.8% p = 0.03; respectively) in relation to basal activity (100.1 +/- 1.6%). In addition, the four peptides were analyzed through in silico analysis, and none of them demonstrated possible hemolytic and toxic activities. In our study, the comprehensive LC-MS and MS/MS analyses of Scolia oculate venom identified four major peptide components of the venom beta-, gamma-, delta- and epsilon-scoliidines, and small differences in their primary structures are important to their neuroprotective properties. (AU) | |
| Processo FAPESP: | 19/20832-7 - Estudo das proteases e peptídeos presentes no veneno do escorpião Tityus serrulatus: aprofundando o conhecimento do veneno e buscando novas terapias para o envenenamento |
| Beneficiário: | Fernanda Calheta Vieira Portaro |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/13124-5 - Purificação e caracterização de peptídeos presentes nos venenos de serpentes africanas: à procura de inibidores de peptidases de importância médica |
| Beneficiário: | Roberto Tadashi Kodama |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |