The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus

Autor(es):Mostrar menos -

Bronzoni, Roberta V. M. ^[1] ; Madrid, Maria C. F. S. ^[1] ; Duarte, Danilo V. B. ^[1] ; Pellegrini, Vanessa O. A. ^{[2, 1]} ; Pacca, Carolina C. ^{[2, 1]} ; Carmo, Ana C. V. ^{[2, 1]} ; Zanelli, Cleslei F. ^[3] ; Valentini, Sandro R. ^[3] ; Santacruz-Perez, Carolina ^{[4, 5]} ; Barbosa, Joao A. R. G. ^{[4, 5]} ; Lutz, Carol S. ^{[6, 7]} ; Rahal, Paula ^[2] ; Nogueira, Mauricio L. ^{[1, 8]}

Afiliação do(s) autor(es)

^[1] Fac Med Rio Preto, Lab Pesquisa Viirol, Dept Doencas Dermatol Infecciosas & Parasitarias, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara - Brazil

^[4] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed 2, BR-05508900 Sao Paulo - Brazil

^[5] Ctr Biol Mol Estrutural, Lab Nacl Luz, BR-6192 Campinas, SP - Brazil

^[6] Univ Med & Dent New Jersey, Dept Biochem & Mol Biol, New Jersey Med Sch, Newark, NJ 07103 - USA

^[7] Univ Med & Dent New Jersey, Grad Sch Biomed Sci, Newark, NJ 07103 - USA

^[8] Fac Med Rio Preto, Lab Pesquisa Viirol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 156, n. 6, p. 931-938, JUN 2011.
• Citações Web of Science: 4

Resumo

The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed. (AU)

• Processo FAPESP: 05/03260-7 - Replicacao do virus da febre amarela: estudo das interacoes entre a proteina ns5 e proteinas celulares.
• Beneficiário: Roberta Vieira de Morais Bronzoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado