Characterization of protoporphyrin IX in experiments with mitochondria isolated from mouse liver

Autor(es)

Mezzacappo, Natasha Ferreira ; Vollet Filho, Jose Dirceu ; Inada, Natalia Mayumi ; Bagnato, Vanderlei Salvador

• Tipo de documento: Artigo Científico
• Fonte: 2024 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC 2024; v. N/A, p. 3-pg., 2024-01-01.

Resumo

Protoporphyrin IX (PpIX) is an intermediate in heme biosynthesis, synthesized within the mitochondria. As a photosensitizer in photodynamic therapy (PDT), PpIX primarily targets mitochondrial structures. This study investigates the interaction of PpIX with mitochondria isolated from mouse liver. Absorbance analysis reveals that exogenous PpIX added to mitochondrial samples in vitro binds significantly to the mitochondria. Fluorescence analysis of mitochondria isolated after delta-aminolevulinic acid (ALA) injection on mice demonstrates that PpIX accumulates within the mitochondria. This is a preliminary study for subsequent in vitro experiments, where PDT will be applied to mitochondria isolated from mouse liver, focusing on analyzing mitochondrial respiration. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático