Synthesis, characterization of europium(III) and terbium(III) complexes containing lawsone ligand and their interaction with DNA, HSA, and topoisomerases

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Rocha, Josias S. ; Pereira, George B. S. ; Palmeira-Mello, Marcos V. ; Oliveira, Willyan F. ; Dutra, Jocely L. ; Teixeira, Tamara ; da Silva, Nadija N. P. ; Oliveira, Gabriela P. ; Gomes-Junior, Paulo C. ; Passaes, Anna C. S. J. ; Araujo-Neto, Joao H. ; Batista, Alzir A. ; Ellena, Javier ; Dutra, Jose D. L. ; Farias, Renan L. ; Rocha, Fillipe, V

• Tipo de documento: Artigo Científico
• Fonte: Journal of Molecular Structure; v. 1333, p. 13-pg., 2025-02-22.

Resumo

In this study, two complexes based on Europium [Eu(LAW)3(H2O)3] (1) and Terbium [Tb(LAW)3(H2O)3] (2), each containing the lawsone ligand, were synthesized and comprehensively characterized using Infrared and UVVisible spectroscopies, Cyclic Voltammetry, Conductivity, and Magnetic Susceptibility measurements. Crystal structure information was determined via X-ray diffraction (XRD), and elemental analysis confirmed the compounds' purity. Experimental and theoretical UV-Vis data were compared through TD-DFT calculations, providing insights into the low luminescence of both Eu(III) and Tb(III) complexes, with excited states calculated both with and without solvent effects. DNA-binding experiments with ct-DNA were conducted using viscosity measurements, UV-Vis titrations, circular dichroism, and fluorescence competition assays to explore the complexes' primary interactions with the DNA target. Additionally, DNA cleavage properties were examined using pBR322 plasmid DNA with agarose gel electrophoresis. Although no cleavage activity was observed, DNA interaction studies suggested covalent binding, supported by viscosity and circular dichroism data. Furthermore, analyzing the docking results, both compounds demonstrated stable conformations within the Sudlow I site also the lawsone-based compounds demonstrated an ability to bind to human serum albumin (HSA) with binding constants (Kb) in the range of 105-106 and showed Topoisomerase II alpha inhibition at 50 mu M. Both compounds remained stable for up to 48 hours. (AU)

• Processo FAPESP: 22/02876-0 - Avaliação do perfil biológico de complexos de coordenação: uma abordagem em modelos celulares 2D e 3D
• Beneficiário: Fillipe Vieira Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/01787-0 - Estudo in vitro e in vivo de complexos fosfínicos de Ru(II) com atividades anticancerígenas
• Beneficiário: Marcos Vinícius Palmeira de Mello
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado