Improved Bioprocess with CHO-hTSH Cells on Higher Microcarrier Concentration Provides Higher Overall Biomass and Productivity for rhTSH

Autor(es)

Ventini, Daniella C. ^{[1, 2]} ; Damiani, Renata ^[3] ; Sousa, Alvaro P. B. ^{[1, 2]} ; de Oliveira, Joao E. ^[3] ; Peroni, Cibele N. ^[3] ; Ribela, Maria T. C. P. ^[3] ; Bartolini, Paolo ^[3] ; Tonso, Aldo ^[2] ; Soares, Carlos R. J. ^[3] ; Pereira, Carlos A. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Imunol Viral, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Celulas Anim, Dept Engn Quim, Escola Politecn, BR-09500900 Sao Paulo - Brazil

^[3] CNEN, IPEN, Ctr Biotecnol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 164, n. 4, p. 401-409, JUN 2011.
• Citações Web of Science: 2

Resumo

Since the recombinant thyroid-stimulating hormone (rhTSH) is secreted by stably transfected Chinese hamster ovary (CHO-hTSH) cells, a bioprocess consisting of immobilizing the cells on a substrate allowing their multiplication is very suitable for rhTSH recovering from supernatants at relative high degree of purity. In addition, such a system has also the advantage of easily allowing delicate manipulations of culture medium replacement. In the present study, we show the development of a laboratory scale bioprocess protocol of CHO-hTSH cell cultures on cytodex microcarriers (MCs) in a 1 L bioreactor, for the preparation of rhTSH batches in view of structure/function studies. CHO-hTSH cells were cultivated on a fetal bovine serum supplemented medium during cell growth phase. For rhTSH synthesis phase, 75% of supernatant was replaced by animal protein-free medium every 24 h. Cell cultures were monitored for agitation (rpm), temperature (A degrees C), dissolved oxygen (% DO), pH, cell concentration, MCs coverage, glucose consumption, lactate production, and rhTSH expression. The results indicate that the amount of MCs in the culture and the cell concentration at the beginning of rhTSH synthesis phase were crucial parameters for improving the final rhTSH production. By cultivating the CHO-hTSH cells with an initial cell seeding of four cells/MC on 4 g/L of MCs with a repeated fed batch mode of operation at 40 rpm, 37 A degrees C, 20% DO, and pH 7.2 and starting the rhTSH synthesis phase with 3 x 10(6) cells/mL, we were able to supply the cultures with enough glucose, to maintain low levels of lactate, and to provide high percent (similar to 80%) of fully covered MCs for a long period (5 days) and attain a high cell concentration (similar to 9 x 10(5) cells/mL). The novelty of the present study is represented by the establishment of cell culture conditions allowing us to produce similar to 1.6 mg/L of rhTSH in an already suitable degree of purity. Batches of produced rhTSH were purified and showed biological activity. (AU)

• Processo FAPESP: 07/52264-0 - Desenvolvimento de bioprocessos com células CHO e S2 em biorreatores para produção em larga escala de proteínas recombinantes
• Beneficiário: Daniella Cristina Ventini Monteiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/60826-3 - Desenvolvimento de bioprocesso para produção de larga escala de hormônios recombinantes e seus antagonistas em cultura de células CHO em biorreatores
• Beneficiário: Carlos Roberto Jorge Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/09482-3 - Expressão de genes heterólogos em células de dípteros: biologia molecular e engenharia de processos
• Beneficiário: Carlos Augusto Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/57794-5 - Desenvolvimento de bioprocesso para producao em larga escala de hormonios recombinantes e seus antagonistas em culturas de celulas cho em biorreatores.
• Beneficiário: Álvaro Paiva Braga de Sousa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado