Biochemical responses of the ethylene-insensitive Never ripe tomato mutant subjected to cadmium and sodium stresses

Autor(es)

Monteiro, Carolina C. ^[1] ; Carvalho, Rogerio F. ^[1] ; Gratao, Priscila L. ^[1] ; Carvalho, Giselle ^[1] ; Tezotto, Tiago ^[1] ; Medici, Leonardo O. ^[2] ; Peres, Lazaro E. P. ^[3] ; Azevedo, Ricardo A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Fed Rural Rio de Janeiro, Dept Ciencias Fisiol, BR-23890000 Seropedica, RJ - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 71, n. 2, p. 306-320, JUN 2011.
• Citações Web of Science: 83

Resumo

In order to further address the known interaction between ethylene and components of the oxidative system, we have used the ethylene-insensitive Never ripe (Nr) tomato (Solanum lycopersicum L) mutant, which blocks ethylene responses. The mutant was compared to the control Micro-Tom (MT) cultivar subjected to two stressful situations: 100 mM NaCl and 0.5 mM CdCl(2). Leaf chlorophyll, lipid peroxidation and antioxidant enzyme activities in roots, leaves and fruits, and Na and Cd accumulation in tissues were determined. Although we verified a similar growth pattern and Na and Cd accumulation for MT and Nr, the mutant exhibited reduced leaf chlorophyll degradation following stress. In roots and leaves, the patterns of catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX), guaiacol peroxidase (GPOX), superoxide dismutase (SOD) enzyme activity as well as malondialdehyde (MDA) and hydrogen peroxide (H(2)O(2)) production under the stressful conditions tested were very similar between MT and Nr mutant. However, Nr fruits showed increased H(2)O(2) production, reduced and enhanced APX activity in NaCl and CdCl(2), respectively, and enhanced GPOX in NaCl. Moreover, through non-denaturing PAGE, a similar reduction of SOD I band intensity in both, control MT and Nr mutant, treated with NaCl was observed. In leaves and fruits, a similar SOD activity pattern was observed for all periods, genotypes and treatments. Overall the results indicate that the ethylene signaling associated with NR receptor can modulate the biochemical pathways of oxidative stress in a tissue dependent manner, and that this signaling may be different following Na and Cd exposure. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/08444-6 - Resposta de plantas a metais pesados: analise dos sistemas antioxidantes e de complexacao a metais.
• Beneficiário: Ricardo Antunes de Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular