| Texto completo | |
| Autor(es): |
Rosa, Jose C.
[1]
;
Lira, Fabio S.
[2, 1]
;
Eguchi, Ricardo
[1]
;
Pimentel, Gustavo D.
[1]
;
Venancio, Daniel P.
[1]
;
Cunha, Claudio A.
[1]
;
Oyama, Lila M.
[1]
;
De Mello, Marco T.
[2]
;
Seelaender, Marilia
[3]
;
Oller Do Nascimento, Claudia M.
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Physiol Nutr, BR-04023060 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Psychobiol, BR-04023060 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Cell & Dev Biol, Canc Metab Res Grp, Inst Biomed Sci 1, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Cellular Physiology; v. 226, n. 6, p. 1604-1607, JUN 2011. |
| Citações Web of Science: | 17 |
| Resumo | |
Cytokines (IL-6, IL-10, and TNF-alpha) are increased after exhaustive exercise in the retroperitoneal adipose tissue (RPAT) and mesenteric adipose tissue (MEAT). An exhaustive acute exercise protocol induces inflammation in adipose tissue that lasts 6 h after the exercise has ended. It is well-established that this protocol increases circulating plasma levels of non-esterified fatty acids (NEFAs) and lipopolysaccharides (LPS), compounds that are important in stimulating signaling via toll like receptor-4 (TLR-4) in different type cells. In the present study, we investigated the regulation of TLR-4 and DNA-binding of nuclear factor-kappa Bp65 (NF-kappa Bp65) in different depots of adipose tissue in rats after exhaustive exercise. Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6), and 6 h (E6 group, n = 6) after the exhaustive exercise, which consisted of running on a treadmill (approximately 70% V(O2max)) for 50 min and then running at an elevated rate that increased at 1 m/min, until exhaustion. The control group (C group, n = 6) was not subjected to exercise. In RPAT, TLR-4, MYD-88, and IkB alpha increased in the E2 group after exercise. MYD-88 and TRAF6 remained increased in the E6 group in comparison with the control group. DNA-binding of NF-kappa Bp65 was not altered. In MEAT, TLR-4, MYD-88, TRAF6, and DNA-binding of NF-kappa Bp65 were increased only in the E6 group. In conclusion, we have shown that increases in pro-inflammatory cytokines in adipose tissue pads after exhaustive exercise may be mediated via TLR-4 signaling, leading to increases in NF-kappa Bp65 binding to DNA in MEAT. J. Cell. Physiol. 226: 1604-1607, 2011. (C) 2010 Wiley-Liss, Inc. (AU) | |
| Processo FAPESP: | 05/01445-0 - Papel dos PPARs na caquexia associada ao câncer |
| Beneficiário: | Marilia Cerqueira Leite Seelaender |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/11588-0 - Expressao de receptores de prostanoides em hepatocitos perioportais e perivenosos: efeitos de mediadores da caquexia associada ao cancer. |
| Beneficiário: | Marilia Cerqueira Leite Seelaender |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |