Stimulation of Acidic Reduction of Nitrite to Nitric Oxide by Soybean Phenolics: Possible Relevance to Gastrointestinal Host Defense

Autor(es)

Ferreira, Pablo Gomes ^[1] ; Suedan Souza Lima, Maria Angelica ^[1] ; Bernedo-Navarro, Robert Alvin ^[2] ; Conceicao, Rogerio Arcuri ^[2] ; Linhares, Edlaine ^[3] ; Helena Frankland Sawaya, Alexandra Christine ^[1] ; Yano, Tomomasa ^[2] ; Salgado, Ione ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Genet & Evolucao & Bioagentes, BR-13083970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05513970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 59, n. 10, p. 5609-5616, MAY 25 2011.
• Citações Web of Science: 7

Resumo

This study aimed to evaluate the potential of soybean-promoted acidic nitrite reduction and to correlate this activity with the content of phenolics and with the bactericidal activity against Escherichia coli O157:H7. Extracts of embrionary axes and cotyledons enriched in phenolics increased (center dot)NO formation at acidic pH at values that were 7.1 and 4.5 times higher, respectively, when compared to the reduction of the nonenriched extracts. Among the various phenolics accumulated in the soybean extracts, five stimulated nitrite reduction in the following decreasing order of potency: epicatechin gallate, chlorogenic acid, caffeic acid, galic acid and p-coumaric acid. Extracts of embrionary axes presented higher contents of epicatechin gallate and caffeic acid, compared to that of cotyledons, indicating a positive correlation between activity of the extracts and content of phenolics with regard to nitrite reducing activity. Soybean extracts enriched in phenolics interacted synergistically with acidified nitrite to prevent E. coli O157:H7 growth. The results suggest that soybean phenolics may interfere with the metabolism of (center dot)NO in an acidic environment by accelerating the reduction of nitrite, with a potential antimicrobial effect in the stomach. (AU)

• Processo FAPESP: 08/11636-5 - Sinalização do óxido nítrico (NO) na resposta de defesa de plantas ao ataque de patógenos: análise da expressão gênica, produção de compostos secundários e bioenergética mitocondrial
• Beneficiário: Ione Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Regular