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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stimulation of Acidic Reduction of Nitrite to Nitric Oxide by Soybean Phenolics: Possible Relevance to Gastrointestinal Host Defense

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Author(s):
Ferreira, Pablo Gomes [1] ; Suedan Souza Lima, Maria Angelica [1] ; Bernedo-Navarro, Robert Alvin [2] ; Conceicao, Rogerio Arcuri [2] ; Linhares, Edlaine [3] ; Helena Frankland Sawaya, Alexandra Christine [1] ; Yano, Tomomasa [2] ; Salgado, Ione [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Genet & Evolucao & Bioagentes, BR-13083970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05513970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Agricultural and Food Chemistry; v. 59, n. 10, p. 5609-5616, MAY 25 2011.
Web of Science Citations: 7
Abstract

This study aimed to evaluate the potential of soybean-promoted acidic nitrite reduction and to correlate this activity with the content of phenolics and with the bactericidal activity against Escherichia coli O157:H7. Extracts of embrionary axes and cotyledons enriched in phenolics increased (center dot)NO formation at acidic pH at values that were 7.1 and 4.5 times higher, respectively, when compared to the reduction of the nonenriched extracts. Among the various phenolics accumulated in the soybean extracts, five stimulated nitrite reduction in the following decreasing order of potency: epicatechin gallate, chlorogenic acid, caffeic acid, galic acid and p-coumaric acid. Extracts of embrionary axes presented higher contents of epicatechin gallate and caffeic acid, compared to that of cotyledons, indicating a positive correlation between activity of the extracts and content of phenolics with regard to nitrite reducing activity. Soybean extracts enriched in phenolics interacted synergistically with acidified nitrite to prevent E. coli O157:H7 growth. The results suggest that soybean phenolics may interfere with the metabolism of (center dot)NO in an acidic environment by accelerating the reduction of nitrite, with a potential antimicrobial effect in the stomach. (AU)

FAPESP's process: 08/11636-5 - Nitric oxide signaling in plant defense response against pathogen attack: analysis of gene expression, production of secundary compounds and mitochondrial bioenergetics
Grantee:Ione Salgado
Support Opportunities: Regular Research Grants