Autor(es)

Leite, Renata de Freitas ; Nery Garcia, Breno Luis ; Barbosa, Kristian da Silva ; Mitsunaga, Thatiane Mendes ; Fidelis, Carlos Eduardo ; Moreira Dias, Bruna Juliana ; de Miranda, Renata Rank ; Zucolotto, Valtencir ; Good, Liam ; dos Santos, Marcos Veiga

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY SCIENCES; v. 12, n. 5, p. 23-pg., 2025-05-21.

Resumo

Staphylococcus aureus is a mastitis pathogen that compromises cow health and causes significant economic losses in the dairy industry. High antimicrobial resistance and biofilm formation by S. aureus limit the efficacy of conventional treatments. This study evaluated the potential of polyhexamethylene biguanide nanoparticles (PHMB NPs) against mastitis-causing S. aureus. PHMB NPs showed low toxicity to bovine mammary epithelial cells (MAC-T cells) at concentrations up to four times higher than the minimum inhibitory concentration (1 mu g/mL) against S. aureus. In Experiment 1, PHMB NPs significantly reduced biofilm formation by S. aureus by 50% at concentrations >= 1 mu g/mL, though they showed limited efficacy against preformed biofilms. In Experiment 2, using an excised teat model, PHMB NPs reduced S. aureus concentrations by 37.57% compared to conventional disinfectants (chlorhexidine gluconate, povidone-iodine, and sodium dichloroisocyanurate), though limited by short contact time. These findings highlight the potential of PHMB NPs for the control of S. aureus growth and biofilm formation. (AU)

• Processo FAPESP: 17/50461-5 - Mastite bovina: controle pelo uso de nanopartículas polyplex administradas localmente
• Beneficiário: Marcos Veiga dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/01705-4 - Eficácia de nanopartículas constituídas por biguanida de polihexametileno para o controle de Staphylococcus aureus causadores de Mastite Bovina
• Beneficiário: Renata de Freitas Leite
• Modalidade de apoio: Bolsas no Brasil - Doutorado