| Texto completo | |
| Autor(es): |
de Souza Pereira, Carolina
;
Mastrochirico-Filho, Vito Antonio
;
Pereira, Elcimara Cardoso
;
Butzge, Arno Juliano
;
de Souza Borges, Carolina Heloisa
;
Caceres, Pablo
;
Porto-Foresti, Fabio
;
Yanez, Jose Manuel
;
Gallani, Silvia Umeda
;
Hashimoto, Diogo Teruo
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MOLECULAR BIOLOGY REPORTS; v. 52, n. 1, p. 16-pg., 2025-12-01. |
| Resumo | |
BackgroundFlavobacterium oreochromis has been associated with elevated mortality rates during the early stages of tambaqui (Colossoma macropomum) aquaculture. This study investigated genetic responses to bacterial infection in juvenile fish by comparing gene expression profiles between symptomatic (IS) and asymptomatic (IA) individuals.Methods and resultsSkin samples from both IA and IS individuals were collected for transcriptome sequencing. Approximately 21 million reads per library were aligned to the tambaqui genome. Differential expression analysis revealed 2,176 upregulated and 1,219 downregulated genes in IS individuals, whereas 1,358 genes were upregulated and 488 downregulated in IA individuals. Notably, genes upregulated in both groups were associated with autophagy (e.g., atg4b and ulk2) and oxidative stress responses (e.g., klf9 and txnip). In contrast, genes related to tissue integrity, such as unc45b and akap6, were consistently downregulated during infection. These results suggest a dual host response to F. oreochromis infection, characterized by activation of cellular stress pathways and the suppression of genes involved in maintaining structural integrity. The upregulation of autophagy-related (atg4b, ulk2) and oxidative stress-regulating (klf9 and txnip) genes likely reflects an attempt by the host to counteract bacterial invasion through enhanced intracellular degradation and redox homeostasis. Conversely, the downregulation of unc45b and akap6 may indicate a compromise in structural defense mechanisms.ConclusionThese findings offer valuable insights into the immunogenetics of tambaqui and have direct implications for enhancing disease resistance in aquaculture. Moreover, they contribute to a better understanding of the complex interplay between molecular pathways involved in F. oreochromis infection. (AU) | |
| Processo FAPESP: | 22/09226-0 - Otimização de ferramentas genômicas para investigar assinaturas de resistência em pacu (Piaractus mesopotamicus) à infecção por Aeromonas hydrophila |
| Beneficiário: | Vito Antonio Mastrochirico Filho |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 20/11049-4 - Base genética para a resistência à bactéria Aeromonas hydrophila em populações de Pacu (Piaractus mesopotamicus) |
| Beneficiário: | Vito Antonio Mastrochirico Filho |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 20/07959-5 - Cadeia produtiva do tambaqui: seleção genômica para resistência/tolerância à acantocefalose |
| Beneficiário: | Diogo Teruo Hashimoto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |