| Texto completo | |
| Autor(es): |
Scortecci, Jessica Fernandes
;
Fernandes, Adriano de Freitas
;
Serrao, Vitor Hugo Balasco
;
Cornelio, Marinonio Lopes
;
Neto, Mario de Oliveira
;
Thiemann, Otavio Henrique
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1873, n. 4, p. 9-pg., 2025-05-02. |
| Resumo | |
The Selenocysteine (Sec-U) biosynthesis pathway is present in eukaryotes and prokaryotes, in which its incorporation is directed by the stop codon UGA and the structural mRNA element named Sec Insertion Sequence (SECIS) that contain an essential kink-turn motif recognized by specific RNA-binding proteins. SBP2 is the key player in the interaction with the SECIS element in eukaryotes, and it is essential for the biosynthesis pathway. Free-living amoebas are part of the Heterolobosea phylum, and several species, including Naegleria fowleri, are known human pathogens. In 2013 it was reported that Naegleria gruberi (NgSBP2), which is non-pathogenic, had a divergent SBP2 sequence and all the genes essential for Sec synthesis. The identity of NgSBP2 is confirmed experimentally and its binding affinity to the SECIS element is demonstrated. The N-terminal and the C-terminal domains (NgSBP2-NT and NgSBP2-CT, respectively) of NgSBP2 contain disordered regions, particularly in the Nterminal domain. The SECIS element is bonded to NgSBP2-CT, which results in a decrease in the disordered sequence of the domain, and the NgSBP2-NT domain interacts with NgSBP2-CT.SECIS complex, as we present here. The findings reveal the molecular interaction patterns underlying the selenocysteine incorporation pathway in an early-branching eukaryote, which is influenced by multiple protein-RNA interactions. (AU) | |
| Processo FAPESP: | 13/17791-0 - Caracterização molecular e estrutural do Leishmania RNA vírus LRV1-4 |
| Beneficiário: | Otavio Henrique Thiemann |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/20977-7 - Estudo da via de incorporação de selenocisteínas: compreensão dos mecanismos de interações macromoleculares |
| Beneficiário: | Jessica Fernandes Scortecci |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 14/16005-4 - Caracterização das interações moleculares entre proteínas da via de síntese de selenocisteínas. |
| Beneficiário: | Jessica Fernandes Scortecci |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 14/00206-0 - Estrutura e função dos peptídeos de fusão da glicoproteína spike do SARS-CoV |
| Beneficiário: | Luís Guilherme Mansor Basso |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/23730-1 - Caracterização das interações macromoleculares das proteínas envolvidas na síntese de selenocisteínas em escherichia coli |
| Beneficiário: | Vitor Hugo Balasco Serrão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |