Umbelliferone induces changes in the structure and pharmacological activities of Bn IV, a phospholipase A(2) isoform isolated from Bothrops neuwiedi

Autor(es)

Toyama, Daniela de Oliveira ^[1] ; dos Santos Diz Filho, Eduardo Britto ^{[2, 3]} ; Cavada, Benildo Sousa ^[4] ; Matias da Rocha, Bruno Anderson ; Buzzo de Oliveira, Simone Cristina ^{[2, 3]} ; Cotrim, Camila Aparecida ^{[2, 3]} ; Gomes Soares, Veronica Cristina ^{[2, 3]} ; Delatorre, Plinio ^[5] ; Marangoni, Sergio ^[2] ; Toyama, Marcos Hikari ^[3]

Afiliação do(s) autor(es)

^[1] Univ Presbiteriana Mackenzie, Ctr Ciencias Biol & Saude, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Bioquim, Inst Biol, Campinas, SP - Brazil

^[3] UNESP, Lab Quim Macromol, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP - Brazil

^[4] Univ Fed Ceara, Dept Bioquim & Biol Mol, Fortaleza, Ceara - Brazil

^[5] Univ Fed Paraiba, Dept Biol Mol, BR-58059900 Joao Pessoa, Paraiba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 57, n. 6, p. 851-860, MAY 2011.
• Citações Web of Science: 13

Resumo

In this paper was demonstrated that umbelliferone induces changes in structure and pharmacological activities of Bn IV, a lysine 49 secretory phospholipase A(2) (sPLA2) from Both tops neuwiedi. Incubation of Bn IV with umbelliferone virtually abolished platelet aggregation, edema, and myotoxicity induced by native Bn IV. The amino acid sequence of Bn IV showed high sequence similarities with other Lys49 sPLA2s from B. jararacussu (BthTx-I), B. pirajai (PrTx-I), and B. neuwiedi pauloensis (Bn SP6 and Bn SP7). This sPLA2 also has a highly conserved C-terminal amino acid sequence, which has been shown as important for the pharmacological activities of Lys49 sPLA2. Sequencing of Bn IV previously treated with umbelliferone revealed modification of S(1) and S(20). Fluorescent spectral analysis and circular dichroism (CD) studies showed that umbelliferone modified the secondary structure of this protein. Moreover, the pharmacological activity of Bn IV is driven by synergism of the C-terminal region with the a-helix motifs, which are involved in substrate binding of the Asp49 and Lys49 residues of 5PLA2 and have a direct effect on the Ca(2+)-independent membrane damage of some secretory snake venom PLA2. For Bn IV, these interactions are potentially important for triggering the pharmacological activity of this 5PLA2. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/54714-3 - Avaliacao da fosfolipase a2 secretorias na infeccao experimental causada por leishmania (leishmania) amazonensis.
• Beneficiário: Marcos Hikari Toyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/55778-2 - Avaliacao dos efeitos induzidos por cumarians sobre a atividade inflamatoria e biologica de pla2 de serpentes bothrops jararacussu e crotalus durissus collilineatus.
• Beneficiário: Marcos Hikari Toyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular