| Texto completo | |
| Autor(es): |
Doonan, Liam B.
;
Lynham, Steven
;
Quinlan, Catherine
;
Ibiji, Spike C.
;
Winter, Carlos E.
;
Padilla, Gabriel
;
Jaimes-Becerra, Adrian
;
Morandini, Andre C.
;
Marques, Antonio C.
;
Long, Paul F.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOLOGICAL BULLETIN; v. 237, n. 1, p. 10-pg., 2019-08-01. |
| Resumo | |
In this quantitative proteomics study we determined the variety and relative abundance of toxins present in enriched preparations of two nematocyst types isolated from the primary tentacles of the adult medusa stage of the hydrozoan Olindias sambaquiensis. The two nematocyst types were microbasic mastigophores and microbasic euryteles, and these were recovered from the macerated tentacle tissues by using a differential centrifugation approach. Soluble protein extracts from these nematocysts were tagged with tandem mass tag isobaric labels and putative toxins identified using tandem mass spectrometry coupled with a stringent bioinformatics annotation pipeline. Astonishingly, the venom composition of the two capsule types was nearly identical, and there was also little difference in the comparative abundance of toxins between the two nematocyst preparations. This homogeneity suggested that the same toxin complement was present regardless of the penetrative ability of the nematocyst type. Predicted toxin protein families that constituted the venom closely matched those of the toxic proteome of O. sambaquiensis published four years previously, suggesting that venom composition in this species changes little over time. Retaining an array of different nematocyst types to deliver a single venom, rather than sustaining the high metabolic cost necessary to maintain a dynamically evolving venom, may be more advantageous, given the vastly different interspecific interactions that adult medusa encounter in coastal zones. (AU) | |
| Processo FAPESP: | 13/50484-4 - Biodiversity and distribution patterns of the Medusozoa form the South-Western Atlantic |
| Beneficiário: | Antonio Carlos Marques |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/50242-5 - Dimensões da vida marinha: padrões e processos de diversificação em cnidários planctônicos e bentônicos |
| Beneficiário: | Antonio Carlos Marques |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |
| Processo FAPESP: | 10/50174-7 - Sistemática, ciclo de vida e padrões reprodutivos de medusas (Cnidaria: Medusozoa: Cubozoa e Scyphozoa) na Baixada Santista (São Paulo, Brasil): biodiversidade marinha |
| Beneficiário: | André Carrara Morandini |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |
| Processo FAPESP: | 10/06927-0 - Biodiversidade e áreas de endemismo de hidroides bentônicos (Cnidaria, Hydrozoa) da costa austral da América do Sul e Antártica |
| Beneficiário: | Thaís Pires Miranda |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 15/21007-9 - Reconhecendo a diversidade de águas-vivas (Medusozoa, Rhopaliophora) |
| Beneficiário: | André Carrara Morandini |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/52324-6 - Síntese e lacunas do conhecimento sobre os organismos da zona costeiro-marinha brasileira |
| Beneficiário: | Antonio Carlos Marques |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |