Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from Sao Paulo state, Brazil

Autor(es)

Araujo-Coutinho, Carlos Jose P. C. ^[1] ; Bernardino, Thaissa C. ^[1] ; Pires, Eder S. ^[2] ; Esch, Lygia V. S. ^[2] ; Viviani, Andrea B. P. ^[3] ; Cavados, Clara F. G. ^[2]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

^[2] Inst Oswaldo Cruz Fiocruz, Lab Fisiol Bacteriana, BR-21040900 Rio De Janeiro - Brazil

^[3] Lab Simulideos, BR-11665070 Caraguatatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Invertebrate Pathology; v. 107, n. 1, p. 11-15, MAY 2011.
• Citações Web of Science: 2

Resumo

The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in Sao Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L(2) fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/05700-2 - Microrganismos Entomopatogênicos para o Manejo Integrado de Dípteros Vetores: Caracterização Molecular, Patologia e Epidemiologia
• Beneficiário: Carlos José Pereira da Cunha de Araújo Coutinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular