| Texto completo | |
| Autor(es): Mostrar menos - |
Pagliuso, D.
;
Grandis, A.
;
Santos, D. G.
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Ferreira, C.
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Terra, W. R.
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Cardoso, C.
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Pimentel, A. C.
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Fuzita, F. J.
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Pereira, J.
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Ramos, I.
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Quezia, L.
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Bahia, A. C.
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Heise, N.
;
Buckeridge, M. S.
;
Machado, E. A.
Número total de Autores: 15
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BioEnergy Research; v. 18, n. 1, p. 12-pg., 2025-07-25. |
| Resumo | |
Cockroaches are omnivorous insects that consume a diverse diet, including lignocellulose. This study investigated Periplaneta americana, which was fed exclusively on sugarcane bagasse, to evaluate biomass degradation across gut chambers and provide helpful information to the bioenergy industry. We analyzed enzyme activity, the composition of monosaccharides from bagasse, and lignin content in gut sections, feces, and food bolus. The fiber's morphology was also evaluated. Bagasse breakdown occurs sequentially (foregut, midgut, hindgut) through mechanical grinding and glycosyl hydrolase activity. The foregut primarily reduces particle size (> 90%) through chewing and enzymatic action, aided by chitinous teeth. Sugarcane bagasse in the midgut resembled the foregut's morphology, while the hindgut and feces displayed microorganisms and small surface holes. The study also explored links between the distribution of monosaccharides and glycosyl hydrolase activity in gut chambers. Monosaccharide profiling revealed elevated levels of rhamnose (similar to 1. 1.2 mu g.mg(-1) CW) and galactose (similar to 5 mu g.mg(-1) CW) in the foregut/midgut, with xylose peaking in the foregut (similar to 15 mu g.mg(-1) CW). Fucose, mannose, arabinose, non-cellulosic, and cellulosic (similar to 1, 4, 4.2, 6, 6.2, 7, and 48 mu g. mg(1) CW, respectively) remained stable across gut regions. Lignin (20% of the cell wall) persisted undigested in bagasse and feces (similar to 20% dry weight). Enzymatic profiling of digestive enzymes showed that the foregut and midgut exhibited higher soluble enzymatic activities against beta Glc (similar to 3), beta Xyl (similar to 1. 8), alpha Xyl (similar to 0. 02), beta Gal (similar to 1.6. 6), alpha Gal (similar to 1), beta Man (similar to 1.3. 3), alpha Ara (similar to 0. 0.8), beta Clb (similar to 0. 8), beta Glca (similar to 0.3. 3), and alpha Rha (similar to 0. 006 mu mol sugar min(-1) mg ptn(-1)). The hindgut (the main microbial chamber) showed lower activity for most enzymes. Fecal analysis indicated digestion/absorption of similar to 50% cellulosic glucose, 25% non-cellulosic glucose, 21% arabinose, and 26% xylose. These findings suggest that P. americana is a promising hemicellulose/cellulose degradation model without lignin breakdown under mesophilic conditions. (AU) | |
| Processo FAPESP: | 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol |
| Beneficiário: | Marcos Silveira Buckeridge |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/13936-0 - O papel do xiloglucano na determinação da arquitetura da parede celular de cana de açúcar |
| Beneficiário: | Adriana Grandis |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 23/18106-1 - Estudo avançado da degradação do bagaço de cana em baratas |
| Beneficiário: | Marcos Silveira Buckeridge |
| Modalidade de apoio: | Auxílio à Pesquisa - Pesquisador Visitante - Brasil |
| Processo FAPESP: | 22/05524-7 - Associação de polissacarídeos na macrofibrila de celulose em parede celular de cana de açúcar |
| Beneficiário: | Adriana Grandis |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |