| Texto completo | |
| Autor(es): |
Torres-Bonilla, Kristian A.
;
Bayona-Serrano, Juan D.
;
Saenz-Suarez, Paula A.
;
Zambrano, Duvan F.
;
Bernal-Bautista, Manuel H.
;
Junqueira-de-Azevedo, Inacio L. M.
;
Hyslop, Stephen
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biochimie; v. 235, p. 12-pg., 2025-08-01. |
| Resumo | |
We describe here the Duvernoy's venom gland organization and biochemical and proteomic analyses of venom from the Colombian colubrid snake Erythrolamprus melanotus. Histological analysis indicated a serous venom gland distinct from the mucous supralabial gland. SDS-PAGE of the venom revealed >15 bands (similar to 15 kDa-similar to 200 kDa) and gelatin zymography revealed potent proteolytic activity; zymographic activity towards casein was considerably less. RP-HPLC yielded 16 peaks, several of which contained proteases, as assessed by gelatin zymography. Enzymatic assays using azocasein, azocoll and hide powder azure confirmed the proteolytic activity. The venom selectively degraded the A alpha-chain of fibrinogen without affecting the B beta and gamma-chains (assessed by SDS-PAGE). All proteolytic activity was inhibited by EDTA (metalloproteinase inhibitor) but not by AEBSF (serine proteinase inhibitor). The venom had no L-amino acid oxidase, esterase, and PLA(2) activities. Proteomic analysis indicated the presence primarily of snake venom metalloproteinases (SVMP), snake venom matrix metalloproteinases (svMMP) and cysteine-rich secretory proteins (CRiSP). SVMP and svMMP were the major components, indicating that this venom is rich in proteases, in agreement with the findings for zymography and enzymatic assays. This study provides the first characterization of Colombian E. melanotus venom and highlights its rich content of metalloproteinases and potent proteolytic activity. (c) 2025 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU) | |
| Processo FAPESP: | 24/14508-0 - Apoio técnico aos projetos de genômica do CeTICS: sequenciamento e montagem de genomas ofídicos |
| Beneficiário: | Juan David Bayona Serrano |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 16/50127-5 - Dimensions US-BIOTA São Paulo: scales of biodiversity: integrated studies of snake venom evolution and function across multiple levels of diversity |
| Beneficiário: | Inácio de Loiola Meirelles Junqueira de Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |
| Processo FAPESP: | 17/24498-9 - Análises venômicas de serpentes Dipsadidae focando em adaptações a ofiofagia |
| Beneficiário: | Juan David Bayona Serrano |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |