| Texto completo | |
| Autor(es): |
Reis, Mouzarllem Barros
;
Bordon, Karla De Castro Figueiredo
;
Martins, Jonas Gama
;
Wiezel, Gisele Adriano
;
Cipriano, Ualter Guilherme
;
Procopio, Rudi Emerson de Lima
;
Bonato, Vania Luiza Deperon
;
Arantes, Eliane Candiani
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN PHARMACOLOGY; v. 16, p. 13-pg., 2025-09-03. |
| Resumo | |
Introduction: Scorpion venoms contain bioactive molecules with potential antitumor properties. This study aimed to evaluate the cytotoxic effects of crude Brotheas amazonicus venom (BamazV) and its molecular weight-separated fractions on human breast cancer cell lines, with a focus on identifying active compounds and elucidating their mechanisms of action. Methods: Human breast epithelial (MCF10A) and breast cancer cell lines (SKBR3, MCF7, and MDA-MB-231) were first assessed for dose-dependent responses to paclitaxel, a standard chemotherapeutic agent. BamazV was fractionated by ultrafiltration into >10 kDa, 3-10 kDa, and <3 kDa fractions, which were tested for cytotoxic activity. The active fraction underwent reversed-phase chromatography, and the major bioactive peptide was characterized by mass spectrometry and Edman degradation. Cytotoxic mechanisms were investigated using cell death assays. Results: All cell lines showed a dose-dependent response to paclitaxel. Crude BamazV induced significant cytotoxicity at concentrations >= 50 mu g/mL, with triple-negative MDA-MB-231 cells being the most sensitive. The >10 kDa fraction retained cytotoxic activity, leading to the isolation of a major peptide, BamazScplp1. Sequence analysis revealed 46-55% identity and 74-81% similarity to known scorpine-like peptides. Functional assays indicated that BamazScplp1 induced predominantly necrotic cell death, consistent with the activity profile of previously reported cytolytic scorpine-like molecules. Discussion: These findings identify BamazScplp1 as a scorpine-like peptide with selective cytotoxicity toward triple-negative breast cancer cells, underscoring the potential of B. amazonicus venom as a source of bioactive compounds for cancer research. (AU) | |
| Processo FAPESP: | 23/01083-9 - Prospecção de peptídeos bioativos com atividade antitumoral isolados de peçonhas de escorpiões da Amazônia Brasileira |
| Beneficiário: | Eliane Candiani Arantes Braga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 23/10400-8 - Prospecção de peptídeos bioativos com atividade antitumoral isolados de peçonhas de escorpiões da Amazônia Brasileira |
| Beneficiário: | Mouzarllem Barros dos Reis |
| Modalidade de apoio: | Bolsas no Brasil - Programa Fixação de Jovens Doutores |
| Processo FAPESP: | 21/11936-3 - Centro de Ciência Translacional e Desenvolvimento de Biofármacos |
| Beneficiário: | Benedito Barraviera |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Ciência para o Desenvolvimento |