| Texto completo | |
| Autor(es): |
Feijoo, Tabata N.
;
Loor-Urgiles, Luis D.
;
de Araujo, Danyelle M.
;
dos Santos, Elisama V.
;
Goulart, Marilia Oliveira Fonseca
;
Martinez-Huitle, Carlos A.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANTIOXIDANTS; v. 14, n. 9, p. 16-pg., 2025-09-03. |
| Resumo | |
Antioxidants, such as flavonoids, are influential secondary metabolites that play a significant role in regulating human health. A & ccedil;a & iacute;, known for its potent antioxidant properties, has gained popularity in the nutritional field. However, there is a need for accurate methods to quantify its antioxidant capacity. Therefore, the goal of this investigation was to determine the total antioxidant capacity of frozen a & ccedil;a & iacute; pulp by applying the concept of the electrochemical quantitative index (EQI) using the cyclic voltammetry technique. The electrochemical response of ethanolic extracts obtained by a nonconventional ultrasound bath was investigated in the anodic region. The results clearly showed redox behavior at +0.37 V and +0.27 V (vs. Ag/AgCl) for the anodic and cathodic peaks, respectively, when evaluated by cyclic voltammetry at a glassy carbon (GC) electrode. By investigating a constant ethanolic extract concentration (0.2%) and analyzing the scan rate and supporting electrolyte effects, it was determined that the frozen a & ccedil;a & iacute; pulp extract presented an EQI of about 2.3 mu A/V. Similarly, the concept of the EQI was extended to the use of the differential pulse voltammetry profile of a 0.2% ethanolic a & ccedil;a & iacute; extract on different supporting electrolytes, which showed that some experimental conditions needed improvement. Still, maintaining pH with a buffer solution in the anodic region is crucial to ensure reproducibility. The antioxidant capacity was also determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay to compare the electrochemical results. The Folin-Ciocalteu colorimetric test was applied to determine the total phenolic content of the extract. (AU) | |
| Processo FAPESP: | 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos |
| Beneficiário: | Maria Valnice Boldrin |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/13113-4 - Processos foto (eletro) catalíticos e fotoeletro-fenton para eliminar contaminantes emergentes de águas de rejeito industriais |
| Beneficiário: | Maria Valnice Boldrin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |