| Texto completo | |
| Autor(es): |
Bordon, Karla C. F.
;
Santos, Gabrielle C.
;
Martins, Jonas G.
;
Wiezel, Gisele A.
;
Amorim, Fernanda G.
;
Crasset, Thomas
;
Redureau, Damien
;
Quinton, Loic
;
Procopio, Rudi E. L.
;
Arantes, Eliane C.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXINS; v. 17, n. 8, p. 23-pg., 2025-08-15. |
| Resumo | |
Scorpionism is a growing public health concern in Brazil, with the Amazon region presenting the highest mortality rates but remaining understudied, especially regarding local scorpion venoms composition. This study presents the first comprehensive biochemical characterization of venoms from three Amazonian species-Tityus metuendus (TmetuV), Tityus silvestris (TsilvV), and Brotheas amazonicus (BamazV)-using an integrated approach combining Multi-Enzymatic Limited Digestion (MELD)-based bottom-up proteomics, high-resolution LC-MS/MS, chromatography, zymography, and enzymatic assays. Tityus serrulatus venom was included as a reference. Significant biochemical differences were observed: TsilvV was rich in 20-30 kDa proteins and showed strong metalloprotease activity; BamazV exhibited high molecular weight proteins and potent phospholipase A(2) (PLA(2)) activity but lacked proteolytic and fibrinogenolytic activities; TmetuV showed the highest hyaluronidase activity and abundance of alpha-KTx neurotoxins. Zymography revealed a conserved similar to 45 kDa hyaluronidase in all species. Three novel components were partially characterized: BamazPLA(2) (Group III PLA(2)), Tmetu1 (37-residue alpha-KTx), and TsilvMP_A (a metalloprotease homologous to antarease). This is the first application of MELD-based proteomics to Amazonian scorpion venoms, revealing molecular diversity and functional divergence within Tityus and Brotheas, emphasizing the need for region-specific antivenoms. These findings provide a foundation for future pharmacological studies and the discovery of bioactive peptides with therapeutic potential. (AU) | |
| Processo FAPESP: | 23/01083-9 - Prospecção de peptídeos bioativos com atividade antitumoral isolados de peçonhas de escorpiões da Amazônia Brasileira |
| Beneficiário: | Eliane Candiani Arantes Braga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/27544-7 - Análise comparativa entre as peçonhas de três espécies de escorpiões da região Amazônica brasileira e Tityus serrulatus: perfil cromatográfico e atividade enzimática |
| Beneficiário: | Gabrielle Cristina Santos |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 21/11936-3 - Centro de Ciência Translacional e Desenvolvimento de Biofármacos |
| Beneficiário: | Benedito Barraviera |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Ciência para o Desenvolvimento |