| Texto completo | |
| Autor(es): Mostrar menos - |
Da Silva, Lucas Henrique Domingos
;
Paris Junior, Jose Alberto
;
Francisco, Rauany Cristina Lopes
;
Trovatti, Eliane
;
Tavares Junior, Alberto Gomes
;
Chorilli, Marlus
;
Haddad, Felipe Falcao
;
Scarim, Caue Benito
;
Floriano, Juliana Ferreira
;
Fardim, Pedro
;
Resende, Flavia Aparecida
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AAPS JOURNAL; v. 28, n. 1, p. 13-pg., 2025-12-17. |
| Resumo | |
Wound healing is a complex process often impaired in severe injuries, requiring innovative therapeutic strategies. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) modulate key cellular pathways, but their clinical application is limited by low stability and bioavailability. This study aimed to evaluate the safety and potential of sodium alginate hydrogels (SAH-EVs) loaded with mesenchymal stem cell-derived extracellular vesicles, focusing on cell migration, cytotoxicity, genotoxicity, and irritation potential. MSC-EVs from Sprague-Dawley rat bone marrow were isolated from conditioned medium collected at 24, 36, 48 and 60 h using size exclusion chromatography and characterized by Nanoparticle Tracking Analysis. The highest EV concentration was obtained from the conditioned medium collected at 36 h, with a main peak at 123 nm. The heterogeneous particle population suggests the presence of EV subtypes. Scanning Electron Microscopy confirmed successful MSC-EVs incorporation into hydrogels with desirable viscoelastic properties. SAH-EVs stimulated HaCaT keratinocyte migration while exhibiting low cytotoxicity in 2D and 3D models, with no genotoxic or mutagenic effects. HET-CAM assays confirmed the absence of irritation potential. These findings highlight the potential of SAH-EVs as a safe biomaterial and lay the groundwork for further investigations into their role in wound healing, reinforcing their relevance in regenerative medicine and tissue engineering. (AU) | |
| Processo FAPESP: | 17/18782-6 - Plataforma click chemistry aplicada ao desenvolvimento de materiais poliméricos para uso em medicina regenerativa |
| Beneficiário: | Eliane Trovatti |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 22/02118-8 - Análise da citotoxicidade e mutagenicidade de materiais de partida e de um novo polímero a base de furano |
| Beneficiário: | Isabela Caroline dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 23/12304-6 - Estudo e Bioengenharia de vesículas extracelulares derivadas do látex natural: novo sistema de entrega de moléculas direcionado com alto potencial para Medicina Regenerativa |
| Beneficiário: | Juliana Ferreira Floriano |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |