| Texto completo | |
| Autor(es): Mostrar menos - |
Mendes Lopes, Alberto Moura
;
Felix de Melo, Allan Henrique
;
Procopio, Dielle Pierroti
;
Teixeira, Gleidson Silva
;
Carazzolle, Marcelo F.
;
de Carvalho, Lucas Miguel
;
Adelantado, Nuria
;
Pereira, Goncalo A. G.
;
Ferrer, Pau
;
Maugeri Filho, Francisco
;
Goldbeck, Rosana
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Process Biochemistry; v. 98, p. 12-pg., 2020-11-01. |
| Resumo | |
Increased demand for biofuels promotes the search for new biomass-degrading fungi. Acremonium strictum is an environmentally widespread filamentous fungi found on plant debris; that secretes lignocellulose-degrading enzymes. A recently isolated A. stricturn strain, AAJ6; native to the Brazilian Cerrado biome was evaluated for its capacity to degrade lignocellulosic substrates. In this study, whole-genome sequencing of AAJ6 was performed and 775 CAZy domains were identified which correlated to those of A. strictum strain DS1bioAY4a and other lignocellulolytic fungi; suggesting AAJ6 is a high CAZyme producer. We expressed the glycoside hydrolase families GH74 and GH3 from plasmid or genome-integrated to evaluate the ethanol production from cellulosic substrates in Brazilian industrial Saccharomyces cerevisiae strains (PE-2 and SA-1) evolved for thermotolerance (AMY12 and AMY35). Those expressing the genome-integrated enzymes showed the highest beta-glucosidase activity and growth in medium with cellobiose at 40 degrees C. The strain AGY005 (integrated cassettes) showed 19, 23 and 46% higher ethanol production in SHF, pSSF (partial hydrolysis SSF) and SSF processes, respectively, using Avicel, and similar to 50% more ethanol using pre-treated sugarcane bagasse, compared to the strain with a plasmid-based expression. These results indicate the improved performance of thermotolerant industrial strains with genome-integrated CAZymes in the SSF process for 2G ethanol. (AU) | |
| Processo FAPESP: | 16/02506-7 - Desenvolvimento de métodos high-throughput para o estudo de quantitative traits loci relacionados à robustez de leveduras industriais (Saccharomyces cerevisiae) |
| Beneficiário: | Gleidson Silva Teixeira |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 16/04602-3 - Desenvolvimento de linhagens de Saccharomyces cerevisiae através da expressão heteróloga de celulases e engenharia evolutiva visando a sacarificação e fermentação simultânea da biomassa lignocelulósica |
| Beneficiário: | Rosana Goldbeck Coelho |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 15/02007-8 - Estudo da expressão heteróloga de celulases de Acremonium strictum em linhagens de Saccharomyces cerevisiae visando a produção de bioetanol de segunda geração |
| Beneficiário: | Dielle Pierotti Procópio |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |