| Texto completo | |
| Autor(es): |
Magalhaes, Caroline A.
[1]
;
Rossato, Sarita S.
[1]
;
Barbosa, Angela S.
[1]
;
dos Santos, Thiago O.
[1]
;
Elias, Waldir P.
[1]
;
Sircili, Marcelo P.
[1]
;
Piazza, Roxane M. F.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Inst Butantan, Bacteriol Lab, Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Memórias do Instituto Oswaldo Cruz; v. 106, n. 2, p. 146-152, MAR 2011. |
| Citações Web of Science: | 7 |
| Resumo | |
Typical and atypical enteropathogenic Escherichia coli (EPEC) are considered important bacterial causes of diarrhoea. Considering the repertoire of virulence genes, atypical EPEC (aEPEC) is a heterogeneous group, harbouring genes that are found in other diarrheagenic E. coli pathotypes, such as those encoding haemolysins. Haemolysins are cytolytic toxins that lyse host cells disrupting the function of the plasma membrane. In addition, these cytolysins mediate a connection to vascular tissue and/or blood components, such as plasma and cellular fibronectin. Therefore, we investigated the haemolytic activity of 72 aEPEC isolates and determined the correlation of this phenotype with the presence of genes encoding enterohaemolysins (Ehly) and cytolysin A (ClyA). In addition, the correlation between the expression of haemolysins and the ability of these secreted proteins to adhere to extracellular matrix (ECM) components was also assessed in this study. Our findings demonstrate that a subset of aEPEC presents haemolytic activity due to the expression of Ehlys and/or ClyA and that this activity is closely related to the ability of these isolates to bind to ECM components. (AU) | |
| Processo FAPESP: | 04/12136-5 - Escherichia coli enteropatogênica atípica (EPEC atípica) |
| Beneficiário: | Waldir Pereira Elias Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 06/07145-0 - ANÁLISE PROTEÔMICA DE ADESINAS DE Escherichia coli ENTEROPATOGÊNICA ATÍPICA |
| Beneficiário: | Roxane Maria Fontes Piazza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 06/58303-5 - Purificacao e caracterizacao de proteina(s) envolvida(s) na adesao de escherichia coli enteropatogenica atipica do sorotipo o26:h11 |
| Beneficiário: | Sarita Schneider Rossato |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |