Bull semen varibles after experimental exposure with Bovine Herpesvirus type 5

Autor(es)

Souza, Diego Gouveia ^[1] ; Silva-Frade, Camila ^{[1, 2]} ; Martins, Jr., Alicio ^[1] ; Cardoso, Tereza C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, UNESP, Lab Anim Reprod, Coll Vet Med, BR-16050680 Sao Paulo - Brazil

^[2] Univ Sao Paulo State, UNESP, Lab Anim Virol, Coll Vet Med, BR-16050680 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 124, n. 1-2, p. 55-60, MAR 2011.
• Citações Web of Science: 3

Resumo

The influence of Bovine Herpesvirus type 5 (BoHV-5) infection on semen variables and sperm morphology collected from healthy bulls with no reproductive disorder was evaluated in ten ejaculates distributed into two experimental groups: group 1, bull semen exposed to 10(2.3) (tissue culture infectious dose) TCID(50)/50 mu l of a Brazilian strain of BoHV-5 (US9/BR/2007; GU9457818) and group II, unexposed bull control semen. After experimental infection, the semen was frozen-thawed prior to computerized analysis (CASA) of sperm motility and movement. Also analyzed were sperm phosphatidylserine transposition, acrosomal integrity, mitochondrial function, plasma membrane integrity and Annexin V expression. Viable BoHV-5 particles and their DNA were detected in infected semen after virus isolation and in situ hybridization (ISH) assay. The ISH revealed the BoHV-5 US9 gene in the acrosome and tail of infected spermatozoa. The only remarkable differences between groups land II were the sperm kinetic variables, whereby infected sperm had a lesser mean velocity (VAP) and curvilinear velocity (VCL) values as compared to controls (P <= 0.05). However, the straightness coefficient (STR) and beat cross frequency (BCF) values were higher in infected sperm. These results indicate that BoHV-5 can be found in infected sperm but induces no functional and morphological damage even after freeze-thawing, and, importantly, BoHV-5 can be spread via in vitro and in vivo reproductive biotechnology procedures. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/57774-7 - Infeccao experimental com herpesvirus bovino tipo 5 e virus da diarreia viral bovina em gametas e embrioes bovinos produzidos in vitro.
• Beneficiário: Camila da Silva Frade
• Modalidade de apoio: Bolsas no Brasil - Mestrado