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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bull semen varibles after experimental exposure with Bovine Herpesvirus type 5

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Author(s):
Souza, Diego Gouveia [1] ; Silva-Frade, Camila [1, 2] ; Martins, Jr., Alicio [1] ; Cardoso, Tereza C. [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo State, UNESP, Lab Anim Reprod, Coll Vet Med, BR-16050680 Sao Paulo - Brazil
[2] Univ Sao Paulo State, UNESP, Lab Anim Virol, Coll Vet Med, BR-16050680 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Animal Reproduction Science; v. 124, n. 1-2, p. 55-60, MAR 2011.
Web of Science Citations: 3
Abstract

The influence of Bovine Herpesvirus type 5 (BoHV-5) infection on semen variables and sperm morphology collected from healthy bulls with no reproductive disorder was evaluated in ten ejaculates distributed into two experimental groups: group 1, bull semen exposed to 10(2.3) (tissue culture infectious dose) TCID(50)/50 mu l of a Brazilian strain of BoHV-5 (US9/BR/2007; GU9457818) and group II, unexposed bull control semen. After experimental infection, the semen was frozen-thawed prior to computerized analysis (CASA) of sperm motility and movement. Also analyzed were sperm phosphatidylserine transposition, acrosomal integrity, mitochondrial function, plasma membrane integrity and Annexin V expression. Viable BoHV-5 particles and their DNA were detected in infected semen after virus isolation and in situ hybridization (ISH) assay. The ISH revealed the BoHV-5 US9 gene in the acrosome and tail of infected spermatozoa. The only remarkable differences between groups land II were the sperm kinetic variables, whereby infected sperm had a lesser mean velocity (VAP) and curvilinear velocity (VCL) values as compared to controls (P <= 0.05). However, the straightness coefficient (STR) and beat cross frequency (BCF) values were higher in infected sperm. These results indicate that BoHV-5 can be found in infected sperm but induces no functional and morphological damage even after freeze-thawing, and, importantly, BoHV-5 can be spread via in vitro and in vivo reproductive biotechnology procedures. (C) 2011 Elsevier B.V. All rights reserved. (AU)