Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata

Autor(es)

Dovigo, Livia Nordi ^[1] ; Pavarina, Ana Claudia ^[1] ; de Oliveira Mima, Ewerton Garcia ^[1] ; Giampaolo, Eunice Teresinha ^[1] ; Vergani, Carlos Eduardo ^[1] ; Bagnato, Vanderlei Salvador ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MYCOSES; v. 54, n. 2, p. 123-130, MAR 2011.
• Citações Web of Science: 92

Resumo

P>Although photodynamic therapy (PDT) has shown great promise for the inactivation of Candida species, its effectiveness against azole-resistant pathogens remains poorly documented. This in vitro study describes the association of Photogem (R) (Photogem, Moscow, Russia) with LED (light emitting diode) light for the photoinactivation of fluconazole-resistant (FR) and American Type Culture Collection (ATCC) strains of Candida albicans and Candida glabrata. Suspensions of each Candida strain were treated with five Photogem (R) concentrations and exposed to four LED light fluences (14, 24, 34 or 50 min of illumination). After incubation (48 h at 37 degrees C), colonies were counted (CFU ml-1). Single-species biofilms were generated on cellulose membrane filters, treated with 25.0 mg l-1 of Photogem (R) and illuminated at 37.5 J cm-2. The biofilms were then disrupted and the viable yeast cells present were determined. Planktonic suspensions of FR strains were effectively killed after PDT. It was observed that the fungicidal effect of PDT was strain-dependent. Significant decreases in biofilm viability were observed for three strains of C. albicans and for two strains of C. glabrata. The results of this investigation demonstrated that although PDT was effective against Candida species, fluconazole-resistant strains showed reduced sensitivity to PDT. Moreover, single-species biofilms were less susceptible to PDT than their planktonic counterparts. (AU)

• Processo FAPESP: 05/03226-3 - Estudo da viabilidade da utilização da terapia fotodinâmica no controle da estomatite protética
• Beneficiário: Ana Cláudia Pavarina
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/02193-4 - Estudo da viabilidade da utilização da terapia fotodinâmica no controle da estomatite protética
• Beneficiário: Ewerton Garcia de Oliveira Mima
• Linha de fomento: Bolsas no Brasil - Doutorado