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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata

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Author(s):
Dovigo, Livia Nordi [1] ; Pavarina, Ana Claudia [1] ; de Oliveira Mima, Ewerton Garcia [1] ; Giampaolo, Eunice Teresinha [1] ; Vergani, Carlos Eduardo [1] ; Bagnato, Vanderlei Salvador [2]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MYCOSES; v. 54, n. 2, p. 123-130, MAR 2011.
Web of Science Citations: 81
Abstract

P>Although photodynamic therapy (PDT) has shown great promise for the inactivation of Candida species, its effectiveness against azole-resistant pathogens remains poorly documented. This in vitro study describes the association of Photogem (R) (Photogem, Moscow, Russia) with LED (light emitting diode) light for the photoinactivation of fluconazole-resistant (FR) and American Type Culture Collection (ATCC) strains of Candida albicans and Candida glabrata. Suspensions of each Candida strain were treated with five Photogem (R) concentrations and exposed to four LED light fluences (14, 24, 34 or 50 min of illumination). After incubation (48 h at 37 degrees C), colonies were counted (CFU ml-1). Single-species biofilms were generated on cellulose membrane filters, treated with 25.0 mg l-1 of Photogem (R) and illuminated at 37.5 J cm-2. The biofilms were then disrupted and the viable yeast cells present were determined. Planktonic suspensions of FR strains were effectively killed after PDT. It was observed that the fungicidal effect of PDT was strain-dependent. Significant decreases in biofilm viability were observed for three strains of C. albicans and for two strains of C. glabrata. The results of this investigation demonstrated that although PDT was effective against Candida species, fluconazole-resistant strains showed reduced sensitivity to PDT. Moreover, single-species biofilms were less susceptible to PDT than their planktonic counterparts. (AU)

FAPESP's process: 05/02193-4 - Study of the viability of the photodynamic therapy on the control of denture stomatitis
Grantee:Ewerton Garcia de Oliveira Mima
Support Opportunities: Scholarships in Brazil - Doctorate