| Texto completo | |
| Autor(es): |
Duque, Cristiane
[1]
;
Stipp, Rafael N.
[1]
;
Wang, Bing
[2]
;
Smith, Daniel J.
[3]
;
Hoefling, Jose F.
[1]
;
Kuramitsu, Howard K.
[2]
;
Duncan, Margaret J.
[4]
;
Mattos-Graner, Renata O.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, BR-13414903 Sao Paulo - Brazil
[2] SUNY Buffalo, Dept Oral Biol, Buffalo, NY 14260 - USA
[3] Forsyth Inst, Dept Immunol, Boston, MA - USA
[4] Forsyth Inst, Dept Mol Genet, Boston, MA - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Infection and Immunity; v. 79, n. 2, p. 786-796, FEB 2011. |
| Citações Web of Science: | 42 |
| Resumo | |
The virulence of the dental caries pathogen Streptococcus mutans relies in part on the sucrose-dependent synthesis of and interaction with glucan, a major component of the extracellular matrix of tooth biofilms. However, the mechanisms by which secreted and/or cell-associated glucan-binding proteins (Gbps) produced by S. mutans participate in biofilm growth remain to be elucidated. In this study, we further investigate GbpB, an essential immunodominant protein with similarity to murein hydrolases. A conditional knockdown mutant that expressed gbpB antisense RNA under the control of a tetracycline-inducible promoter was constructed in strain UA159 (UACA2) and used to investigate the effects of GbpB depletion on biofilm formation and cell surface-associated characteristics. Additionally, regulation of gbpB by the two-component system VicRK was investigated, and phenotypic analysis of a vicK mutant (UAvicK) was performed. GbpB was directly regulated by VicR, and several phenotypic changes were comparable between UACA2 and UAvicK, although differences between these strains existed. It was established that GbpB depletion impaired initial phases of sucrose-dependent biofilm formation, while exogenous native GbpB partially restored the biofilm phenotype. Several cellular traits were significantly affected by GbpB depletion, including altered cell shape, decreased autolysis, increased cell hydrophobicity, and sensitivity to antibiotics and osmotic and oxidative stresses. These data provide the first experimental evidence for GbpB participation in sucrose-dependent biofilm formation and in cell surface properties. (AU) | |
| Processo FAPESP: | 02/07156-1 - Análise da resposta imunológica de crianças durante a colonização por Streptococcus mutans e dos fatores genético-moleculares envolvidos na formação do biofilme dental por estes microrganismos |
| Beneficiário: | Renata de Oliveira Mattos Graner |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 05/55775-0 - Análise genética da função biológica da proteína glucan-binding protein B (GbpB) de Streptococcus mutans |
| Beneficiário: | Cristiane Duque |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 07/56100-2 - Avaliação genética da função biológica da proteína GbpB (glucan binding protein-B) de Streptococcus mutans |
| Beneficiário: | Renata de Oliveira Mattos Graner |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 06/55933-8 - Efeito da inativação de genes regulatórios e da exposição a macrófagos murinos na expressão de glucosiltransferases B e C e de glucan-binding protein B em isolados de Streptococcus mutans |
| Beneficiário: | Rafael Nobrega Stipp |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |