Transenamel and transdentinal cytotoxicity of carbamide peroxide bleaching gels on odontoblast-like MDPC-23 cells

Autor(es)

Soares, D. G. S. ^[1] ; Ribeiro, A. P. D. ^[1] ; Sacono, N. T. ^[2] ; Coldebella, C. R. ^[2] ; Hebling, J. ^[2] ; de Souza Costa, C. A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, Araraquara Sch Dent, Dept Orthodont & Paediat Dent, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Estadual Paulista, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 44, n. 2, p. 116-125, FEB 2011.
• Citações Web of Science: 22

Resumo

P>Aim To evaluate the transenamel and transdentinal cytotoxicity of bleaching gels based on carbamide peroxide (CP) on odontoblast-like cells after different contact times of the products with enamel. Methodology Enamel/dentine discs were obtained from bovine incisors and placed in artificial pulp chambers. Bleaching gels containing 10% or 16% CP were applied for 8 h day-1 on the enamel side of the discs during periods of 1, 7 or 14 days. Deionized water and artificial saliva served as controls. The extracts (culture medium plus bleaching gel products that diffused through the discs) were collected and applied on previously cultured MDPC-23 cells for 1 h. Cell metabolism was evaluated by the MTT assay, and the data were analysed statistically by one-way anova and Tukey's test (alpha = 0.05). Cell morphology was analysed by SEM. Results There was no significant difference (P > 0.05) between the controls and the groups bleached with 10% CP gel. In the groups bleached with 16% CP gel, however, cell metabolism decreased significantly (P < 0.05) by 40.32%, 30.16% and 26.61% at 1, 7 and 14 days, respectively. There was no significant difference (P > 0.05) between 1, 7 or 14 applications of the gels for either of the CP concentrations. Conclusion Regardless of the number of applications on an enamel surface, the 10% CP bleaching gel did not cause transenamel and transdentinal cytotoxicity to the MDPC-23 cell cultures. However, diffusion of products from the 16% CP gel through enamel and dentine and cytopathic effects to the pulp cells occurred even after a single application of this product on enamel. (AU)

• Processo FAPESP: 09/54315-7 - Efeito citotóxico trans-amelodentinario de um gel clareador com 35% de peróxido de hidrogênio aplicado sobre dentes restaurados ou não com resina composta
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular