Development and validation of a simple and rapid capillary zone electrophoresis method for determination of nnrti nevirapine in pharmaceutical formulations

Autor(es)

Zanolli Filho, Luiz A. ^[1] ; Galdez, Cristiane R. ^[2] ; Silva, Claudinei A. ^[1] ; Tavares, Marina F. M. ^[1] ; Costa, Diana M. ^[2] ; Aurora-Prado, Maria S. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Maranhao, Ctr Ciencias Biol & Saude, BR-65085580 Sao Luis, MA - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 22, n. 10, p. 2005-2012, 2011.
• Citações Web of Science: 0

Resumo

A simple and fast capillary zone electrophoresis (CZE) method has been developed and validated for quantification of a non-nucleoside reverse transcriptase inhibitor (NNRTI) nevirapine, in pharmaceuticals. The analysis was optimized using 10 mmol L-1 sodium phosphate buffer pH 2.5, +25 kV applied voltage, hydrodynamic injection 0.5 psi for 5 s and direct UV detection at 200 µm. Diazepam (50.0 µg mL-1) was used as internal standard. Under these conditions, nevirapine was analyzed in approximately less than 2.5 min. The analytical curve presented a coefficient of correlation of 0.9994. Limits of detection and quantification were 1.4 µg mL-1 and 4.3 µg mL-1, respectively. Intra- and inter-day precision expressed as relative standard deviations were 1.4% and 1.3%, respectively and the mean recovery was 100.81%. The active pharmaceutical ingredient was subjected to hydrolysis (acid, basic and neutral) and oxidative stress conditions. No interference of degradation products and tablet excipients were observed. This method showed to be rapid, simple, precise, accurate and economical for determination of nevirapine in pharmaceuticals and it is suitable for routine quality control analysis since CE offers benefits in terms of quicker method development and significantly reduced operating costs. (AU)

• Processo FAPESP: 97/01320-4 - Estratégias para aumento de sensibilidade em eletroforese capilar
• Beneficiário: Marina Franco Maggi Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/52079-3 - Desenvolvimento de metodologias alternativas para o controle de qualidade de anti-retrovirais em medicamentos utilizando eletroforese capilar.
• Beneficiário: Luiz Antonio Zanolli Filho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 00/04414-4 - Técnicas miniaturizadas de separação e análise aplicadas a problemas de impacto clínico/forense, cosmiátrico, ambiental e alimentos
• Beneficiário: Marina Franco Maggi Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Regular