Diagnostic model based on Raman spectra of normal, hyperplasia and prostate adenocarcinoma tissues in vitro

Autor(es)

Lopes, Ricardo Matias ^[1] ; Silveira, Jr., Landulfo ^[2] ; Silva, Marcos Augusto R. S. ^[1] ; Leite, Katia Ramos M. ^[3] ; Pasqualucci, Carlos Augusto G. ^[4] ; Pacheco, Marcos Tadeu T. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Res & Dev, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Camilo Castelo Branco, Grp Biomed Engn, BR-12247004 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Sao Paulo, Dept Urol, Sch Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Pathol, Sch Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROSCOPY-AN INTERNATIONAL JOURNAL; v. 25, n. 2, p. 89-102, 2011.
• Citações Web of Science: 2

Resumo

This study evaluated the use of Raman spectroscopy to identify the spectral differences between normal (N), benign hyperplasia (BPH) and adenocarcinoma (CaP) in fragments of prostate biopsies in vitro with the aim of developing a spectral diagnostic model for tissue classification. A dispersive Raman spectrometer was used with 830 nm wavelength and 80 mW excitation. Following Raman data collection and tissue histopathology (48 fragments diagnosed as N, 43 as BPH and 14 as CaP), two diagnostic models were developed in order to extract diagnostic information: the first using PCA and Mahalanobis analysis techniques and the second one a simplified biochemical model based on spectral features of cholesterol, collagen, smooth muscle cell and adipocyte. Spectral differences between N, BPH and CaP tissues, were observed mainly in the Raman bands associated with proteins, lipids, nucleic and amino acids. The PCA diagnostic model showed a sensitivity and specificity of 100%, which indicates the ability of PCA and Mahalanobis distance techniques to classify tissue changes in vitro. Also, it was found that the relative amount of collagen decreased while the amount of cholesterol and adipocyte increased with severity of the disease. Smooth muscle cell increased in BPH tissue. These characteristics were used for diagnostic purposes. (AU)

• Processo FAPESP: 09/01788-5 - Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata
• Beneficiário: Landulfo Silveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular