Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism

Autor(es)

Coutinho, Tania Alen ^[1] ; Imada, Yumiko ^[2] ; Santos Neves de Barcellos, David Emilio ^[3] ; de Oliveira, Sergio Jose ^[4] ; Moreno, Andrea Micke ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Sanidade Suina & Virol, FMVZ, BR-05508270 Sao Paulo - Brazil

^[2] Natl Inst Anim Hlth, Dept Biol Prod, Tsukuba, Ibaraki 3050856 - Japan

^[3] Univ Fed Rio Grande Sul UFRGS, Setor Suinos, Fac Vet FAVET, BR-91540000 Porto Alegre, RS - Brazil

^[4] Univ Luterana Brasil ULBRA, Lab Microbiol Clin & Alimentos, Fac Vet, BR-92425900 Sao Jose Dos Campos, Canoas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microbiological Methods; v. 84, n. 1, p. 27-32, JAN 2011.
• Citações Web of Science: 2

Resumo

One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP). Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/55502-7 - Caracterização de amostras de Erysipelothrix spp isoladas de suínos nos últimos trinta anos
• Beneficiário: Tania Alen Coutinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado