Differences between salivary and blood neutrophils from elderly and young denture wearers

Autor(es)

Gasparoto, T. H. ^[1] ; Vieira, N. A. ^[2] ; Porto, V. C. ^[3] ; Campanelli, A. P. ; Lara, V. S. ^[4]

Afiliação do(s) autor(es)

^[1] USP, Dept Biol Sci, Bauru Sch Dent, FOB, BR-17012901 Bauru, SP - Brazil

^[2] USP, Clin Anal Lab, Bauru Sch Dent, Hosp Rehabil Craniofacial Anomalies, BR-17012901 Bauru, SP - Brazil

^[3] USP, Dept Prosthodont, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[4] USP, Dept Stomatol Oral Pathol, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Oral Rehabilitation; v. 38, n. 1, p. 41-51, JAN 2011.
• Citações Web of Science: 12

Resumo

We previously showed that neutrophils from patients with Candida-related denture stomatitis exhibited damaged function, and the advance in age intensified this condition. Because such alterations had been determined in elderly people that were not denture wearers, the purpose of this study was to clarify functional and phenotypic characteristics of neutrophils from elderly denture wearers (EDW) and young denture wearers (YDW) without oral lesion. We enrolled 20 denture wearers (12 EDW and 8 YDW) and determined the positivity of Candida species on maxillary prosthesis and palate. Additionally, blood and salivary neutrophils were evaluated. Furthermore, cytokines and chemokines salivary levels were detected. YDW presented higher positivity of Candida albicans than elderly ones. However, blood neutrophils from EDW expressed less CXCR1, CD62L and CD11b and had lower C. albicans phagocytosis than YDW. Although myeloperoxidase and elastase activity was significantly higher in C. albicans-stimulated blood neutrophils from elderly, they produced high levels of IL-10 and low levels of Granulocyte macrophage-colony stimulating factor (GM-CSF). Despite apoptosis rate of salivary neutrophils was enhanced, these cells were at a high number in YDW. GM-CSF and IL10 were lower in saliva from elderly group. These data confirmed that ageing affects blood and salivary neutrophils and could predispose elderly to persistent oral infections. (AU)

• Processo FAPESP: 05/60668-9 - Estudo da função dos neutrófilos nos mecanismos de defesa contra a estomatite por dentadura em indivíduos idosos
• Beneficiário: Thaís Helena Gasparoto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/59612-1 - Senescencia e estomatite por dentadura: avaliacao quantitativa, funcional e fenotipica dos neutrofilos.
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular