Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools

Autor(es)

Verbi Pereira, Fabiola Manhas ^[1] ; Bastos Pereira Milori, Debora Marcondes ^[1] ; Venancio, Andre Leonardo ^[2] ; Tavares Russo, Mariana de Sa ^[1] ; Martins, Polyana Kelly ^[3] ; Freitas-Astua, Juliana ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentacao Agropecuaria, BR-13561206 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis & Matemat, BR-14040901 Ribeirao Preto 3900, SP - Brazil

^[3] Ctr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil

^[4] Embrapa Mandioca & Fruticultura Trop, BR-44380000 Cruz Das Almas, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 83, n. 2, p. 351-356, DEC 15 2010.
• Citações Web of Science: 31

Resumo

This study investigated the organic and inorganic constituents of healthy leaves and Candidatus Liberibacter asiaticus (CLas)-inoculated leaves of citrus plants. The bacteria CLas are one of the causal agents of citrus greening (or Huanglongbing) and its effect on citrus leaves was investigated using laser-induced breakdown spectroscopy (LIBS) combined with chemometrics. The information obtained from the LIBS spectra profiles with chemometrics analysis was promising for the construction of predictive models to identify healthy and infected plants. The major, macro- and microconstituents were relevant for differentiation of the sample conditions. The models were then applied to different inoculation times (from 1 to 8 months). The models were effective in the classification of 82-97% of the diseased samples with a 95% significance level. The novelty of this method was in the fingerprinting of healthy and diseased plants based on their organic and inorganic contents. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/08618-2 - Utilização de imagens de fluorescência, espectroscopia com plasma induzido por laser e quimiometria no estudo da doença Greening em plantações de Citrus
• Beneficiário: Fabiola Manhas Verbi Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado