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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools

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Author(s):
Verbi Pereira, Fabiola Manhas [1] ; Bastos Pereira Milori, Debora Marcondes [1] ; Venancio, Andre Leonardo [2] ; Tavares Russo, Mariana de Sa [1] ; Martins, Polyana Kelly [3] ; Freitas-Astua, Juliana [3, 4]
Total Authors: 6
Affiliation:
[1] Embrapa Instrumentacao Agropecuaria, BR-13561206 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis & Matemat, BR-14040901 Ribeirao Preto 3900, SP - Brazil
[3] Ctr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[4] Embrapa Mandioca & Fruticultura Trop, BR-44380000 Cruz Das Almas, BA - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Talanta; v. 83, n. 2, p. 351-356, DEC 15 2010.
Web of Science Citations: 31
Abstract

This study investigated the organic and inorganic constituents of healthy leaves and Candidatus Liberibacter asiaticus (CLas)-inoculated leaves of citrus plants. The bacteria CLas are one of the causal agents of citrus greening (or Huanglongbing) and its effect on citrus leaves was investigated using laser-induced breakdown spectroscopy (LIBS) combined with chemometrics. The information obtained from the LIBS spectra profiles with chemometrics analysis was promising for the construction of predictive models to identify healthy and infected plants. The major, macro- and microconstituents were relevant for differentiation of the sample conditions. The models were then applied to different inoculation times (from 1 to 8 months). The models were effective in the classification of 82-97% of the diseased samples with a 95% significance level. The novelty of this method was in the fingerprinting of healthy and diseased plants based on their organic and inorganic contents. (C) 2010 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 07/08618-2 - Use of fluorescence images, laser-induced breakdown spectroscopy and chemometrics strategies to Greening disease study in citrus plantation
Grantee:Fabiola Manhas Verbi Pereira
Support Opportunities: Scholarships in Brazil - Post-Doctoral