Genome editing aimed at QTLs validation associated with tolerance Candidatus Liberibacter asiaticus in citrandarins (Citrus sunki x Poncirus trifoliata)

• Grant number: 16/22133-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2017
• Effective date (End): April 30, 2021
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcos Antonio Machado
• Grantee: Maiara Curtolo
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

Despite advances in Brazilian citriculture, propitiated by the development of biotechnological tools, the pests and diseases are still considered as the main obstacles in citrus crop. The huanglongbing (HLB) or greening does not present effective sources of control and resistance causing big damages to the citrus industry.Thus the pursuit of knowledge of genetic mechanisms that trigger many biological processes involved in the response to the HLB is important for the citrus industry. In this sense, the project objectives are: identify genomic regions associated with tolerance to the bacterium Ca. Liberibacter spp through the analysis of expression QTL (eQTL) using linkage maps previously built for P. trifoliata (L.) Raf. cv. Rubidoux using a population of F1 hybrids between 75 Sunki Tangerine and Poncirus trifoliata and assessing the effect of selected genes using genome editing system CRISPR-mediated / CAS9. Application of this technology will increase the association gene x genotype, the knowledge of mechanisms involved in the response to HLB and may contribute with new search strategies of tolerance or resistance for use in susceptible genotypes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SORATTO, TATIANY APARECIDA TEIXEIRA; CURTOLO, MAIARA; MARENGO, SAMANTA; DEZOTTI, ANA LUCIA; LIMA, ROMULO PEDRO MACEDO; GAZAFFI, RODRIGO; MACHADO, MARCOS ANTONIO; CRISTOFANI-YALY, MARIANGELA. QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins. TROPICAL PLANT PATHOLOGY, v. 45, n. 6, SI, MAY 2020. (16/22133-0, 14/50880-0, 11/18605-0, 18/00133-4)

MAIARA CURTOLO; LAÍS MOREIRA GRANATO; TATIANY APARECIDA TEIXEIRA SORATTO; MAISA CURTOLO; RODRIGO GAZAFFI; MARCO AURÉLIO TAKITA; MARIÂNGELA CRISTOFANI-YALY; MARCOS ANTONIO MACHADO. Expression Quantitative Trait Loci (eQTL) mapping for callose synthases in intergeneric hybrids of Citrus challenged with the bacteria Candidatus Liberibacter asiaticus. GENETICS AND MOLECULAR BIOLOGY, v. 43, n. 2, 2020-06-15. (19/01901-8, 14/50880-0, 16/22133-0)

SORATTO, TATIANY APARECIDA TEIXEIRA; CURTOLO, MAIARA; MARENGO, SAMANTA; DEZOTTI, ANA LUCIA; LIMA, ROMULO PEDRO MACEDO; GAZAFFI, RODRIGO; MACHADO, MARCOS ANTONIO; CRISTOFANI-YALY, MARIANGELA. QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins. ROPICAL PLANT PATHOLOG, v. 45, n. 6, p. 20-pg., 2020-05-29. (14/50880-0, 16/22133-0, 18/00133-4, 11/18605-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CURTOLO, Maiara. Target identification strategies and sweet orange (Citrus sinensis L. Osb.) genome editing for tolerance to Huanglongbing. 2021. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.